ISBN-10:
1904455395
ISBN-13:
9781904455394
Pub. Date:
01/01/2009
Publisher:
Caister Academic Press
Real-Time PCR: Current Technology and Applications

Real-Time PCR: Current Technology and Applications

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Overview

Real-Time PCR: Current Technology and Applications

Real-time PCR (RT-PCR) technology is highly flexible and many alternative instruments and fluorescent probe systems have been developed recently. The decreased hands-on time, increased reliability, and improved quantitative accuracy of RT-PCR methods have contributed to the adoption of RT-PCR for a wide range of new applications. This essential manual presents a comprehensive guide to the most up-to-date technologies and applications, as well as providing an overview of the theory of this increasingly important technique. Renowned experts in the field describe and discuss the latest PCR platforms, fluorescent chemistries, validation software, data analysis, and internal and external controls. This timely and authoritative volume also discusses a wide range of RT-PCR applications including clinical diagnostics, biodefense, RNA expression studies, validation of array data, mutation detection, food authenticity and legislation, NASBA, molecular halotyping, and much more. Real-Time PCR: Current Technology and Applications will be an essential book for all laboratories using PCR.

Product Details

ISBN-13: 9781904455394
Publisher: Caister Academic Press
Publication date: 01/01/2009
Pages: 290
Sales rank: 1,183,833
Product dimensions: 7.10(w) x 9.90(h) x 0.90(d)

Table of Contents

List of contributors v

Preface ix

1 An Introduction to Real-Time PCR Nick A. Saunders 1

2 An Overview of PCR Platforms Julie M. J. Logan Kirstin J. Edwards 7

3 Homogeneous Fluorescent Chemistries for Real-Time PCR Martin A. Lee David J. Squirrell Dario L. Leslie Tom Brown 23

4 Reference Gene Validation Software for Improved Normalization Jo Vandesompele Mikael Kubista Michael W. Pfaffl 47

5 Data Analysis Software Michael W. Pfaffl Jo Vandesompele Mikael Kubista 65

6 Performing Real-time PCR Kirstin J. Edwards Julie M. J. Logan 85

7 Internal and External Controls for Reagent Validation Martin A. Lee Dario L. Leslie David J. Squirrell 95

8 Introduction to the Applications of Real-Time PCR Nick A. Saunders 105

9 Analysis of mRNA Expression by Real-time PCR Stephen A. Bustin Tania Nolan 111

10 Validation of Array Data Elisa Wurmbach 137

11 Mutation Detection by Real-Time PCR Elaine Lyon Rong Mao Jeffrey Swensen 149

12 Real-Time NASBA Julie D. Fox Catherine Moore Diana Westmoreland 163

13 Applications in Clinical Microbiology Andrew David Sails 177

14 Diagnosis of Invasive Fungal Infections David S. Perlin 221

15 Biodefence Christina Egan Nick M. Cirino Kimberlee A. Musser 235

16 Real-time PCR: Application to Food Authenticity and Legislation Gordon Wiseman 253

17 Molecular Haplotyping by Real-time PCR Genevieve Pont-Kingdon Alison Millson Elaine Lyon 269

Index 279

Plates A-1

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