Somatic Stem Cells: Methods and Protocols

Somatic Stem Cells: Methods and Protocols

by Shree Ram Singh (Editor)

Paperback(Softcover reprint of the original 1st ed. 2012)

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This Methods in Molecular Biology™ volume offers selected genetic, molecular, and cellular techniques used in somatic stem cell research and its clinical application. Includes materials and reagent lists, step-by-step prools, troubleshooting advice and more.

Product Details

ISBN-13: 9781493962341
Publisher: Springer-Verlag New York, LLC
Publication date: 08/23/2016
Series: Methods in Molecular Biology , #879
Edition description: Softcover reprint of the original 1st ed. 2012
Pages: 546
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

Table of Contents…
Contributing Authors…

Part I Introduction

1. An Update on the Therapeutic Potential of Stem Cell
Pranela Rameshwar, Caitlyn A. Moore, Niloy N. Shah, and Caroline P. Smith

Part II Stem Cell Study in Model Organisms and Human

2. Single-Step Plasmid Based Reprogramming of Human Dermal Fibroblasts to Induced Neural Stem Cells
Luis Azmitia and Philipp Capetian

3. Isolation and Analysis of Mesenchymal Progenitors of the Adult Hematopoietic Niche
Mayra Garcia, Lihong Weng, Xingbin Hu, and Ching-Cheng Chen

4. Identification and Isolation of Mice and Human Hematopoietic Stem Cells
Bijender Kumar and Srideshikan Sargur Madabushi

5. Identification and Characterization of Hair Follicle Stem Cells
Xiaoyang Wang, Yizhan Xing, and Yuhong Li

6. Methods of Mesenchymal Stem Cell Homing to the Blood-Brain Barrier
Peter Conaty, Lauren S. Sherman, Yahaira Naaldijk, Henning Ulrich, Alexandra Stolzing, and Pranela Rameshwar

7. 3D Bioprinting and Stem Cells
Caitlyn A. Moore, Niloy N. Shah, Caroline P. Smith, and Pranela Rameshwar

8. Characterization of Gastrospheres Using 3D Coculture System
Carlos Antônio do Nascimento Santos, Radovan Borojevic, Luiz Eurico Nasciutti, and Christina MaedaTakiy

9. Markers and Methods to Study Intestinal Stem Cells
Nathan Pinto, Beyoncé Carrington, Catharine Dietrich, and Shree Ram Singh

10. Quantitative Analysis of Intestinal Stem Cell Dynamics using Microfabricated Cell Culture Arrays
Leigh A. Samsa, Ian A. Williamson, and Scott T. Magness

11. Detection, Labeling, and Culture of Lung Stem and Progenitor Cells
Ivan Bertoncello, Gianni Carraro, and Jonathan L. McQualter

12. Isolation, Characterization, and Differentiation of Mouse Cardiac Progenitor Cells
Santosh Kumar Yadav and Paras Kumar Mishra

13. Isolating and Characterizing Adipose-Derived Stem Cells
Guangpeng Liu and Xi Chen

14. Enzyme-Free Isolation of Adipose-Derived Mesenchymal Stem Cell
Lauren S. Sherman, Alexandra Condé-Green, Vasanth S. Kotamarti, Edward S. Lee, and Pranela Rameshwar

15. Identification and Characterizations of Annulus Fibrosus-Derived Stem Cells
Qianping Guo, Pinghui Zhou, and Bin Li

16. The Maintenance of Tendon Stem/Progenitor Cells in Culture
Jianying Zhang and James H-C. Wang

17. Intravital Imaging to Understand Spatiotemporal Regulation of Osteogenesis and Angiogenesis in Cranial Defect Repair and Regeneration
Xinping Zhang

18. Beating Heart Cells from Hair Cells
Robert M. Hoffman and Yasuyuki Amoh

19. Generation of FLIP and FLIP-FlpE Targeting Vectors for Bi-Allelic Conditional and Reversible Gene Knockouts in Mouse and Human Cells
Bon-Kyoung Koo

20. Analytical Platforms and Techniques to Study Stem Cells Metabolism
Christine Tang, Kevin Chen, Aleksandar Bajic, William T. Choi, Dodge L. Baluya, and Mirjana Maletic-Savatic

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