Surrogate Tissue Analysis: Genomic, Proteomic, and Metabolomic Approaches

by Michael E. Burczynski (Editor), John C. Rockett (Editor)

Overview

Both an introduction and a fundamental reference, this book explores how to apply genomic, proteomic, and metabolomic technologies to surrogate tissue analysis. It reviews fundamental analytical issues and actual initial results generated using these technologies. The book covers transcriptional profiling (genomics), protein profiling (proteomics), methylation profiling (epigenomics), metabolite profiling (metabolomics) and additional biomarker identification methods that can be applied to surrogate tissues. This book is useful to researchers and scientists interested in applying novel methodologies for identifying biomarkers in the fields of pharmacology, toxicology, and medicine.

Product Details

ISBN-13:	9781040168264
Publisher:	CRC Press
Publication date:	11/10/2005
Sold by:	Barnes & Noble
Format:	eBook
Pages:	332
File size:	7 MB

About the Author

Michael E. Burczynski, John C. Rockett

Table of Contents

INTRODUCTION TO SURROGATE TISSUE ANALYSIS. Introduction to Surrogate Tissue Analysis. GENOMIC APPROACHES. Impact of Sample Handling and Preparation on Gene Signatures as Exemplified for Transcriptome Analysis of Peripheral Blood. Blood Genomic Fingerprints of Brain Diseases. Transcriptional Profiling of Peripheral Blood in Oncology. Blood-Derived Transcriptomic Profiles as a Means to Monitor Levels of Toxicant Exposure and the Effects of Toxicants on Inaccessible Target Tissues. Spermatozoal RNAs as Surrogate Markers of Paternal Exposure. PROTEOMIC APPROACHES. Proteomic Analysis of Surrogate Tissues: Mass Spectrometry-Based Profiling of the Circulatory Proteome for Cancer Detection and Stratification. Lymphocyte Integrins: Potential Surrogate Biomarkers for Evaluation of Endometrial Receptivity. Nipple Aspirate Fluid to Diagnose Breast Cancer and Monitor Response to Treatment. METABOLOMICS AND OTHER APPROACHES. Metabonomics: Metabolic Profiling and Pattern Recognition Analysis of Body Fluids and Tissues for Characterization of Drug Toxicity and Disease Diagnosis. Comprehensive Metabolomic Profiling of Serum and Cerebrospinal Fluid: Understanding Disease, Human Variability, and Toxicity. Lipidomic Analysis of Plasma and Tissues: Lipid-Derived Mediators of Inflammation and Markers of Disease. Molecular Detection and Characterization of Circulating Tumor Cells and Micrometastases in Solid Tumors. Methylation Profiling of Tumor Cells and Tumor DNA in Blood, Urine, and Body Fluids for Cancer Detection and Monitoring. FUTURE CONSIDERATIONS FOR SURROGATE TISSUE PROFILING. Regulatory and Technical Challenges in Incorporating Surrogate Tissue Profiling Strategies into Clinical Development Programs. Considerations in the Economic Assessment of the Value of Molecular Profiling. The Impact and Challenges of Pan-Omic Approaches in Pharmaceutical Discovery and Development. Current and Future Aspects of Surrogate Tissue Analysis. References.

Editorial Reviews

"In summary, the editors and their coauthors have done a very

creditable job of expressing the breadth of the field of surrogate

tissue analysis and provide many concrete examples of techniques

for analysis. The treatment of this arising technology is

at once learned and practical with examples of success and of

the need for further research. The chapters at the end with perspective setting essays on economic and regulatory needs may

help some not accustomed to these issues to understand the issues.

However, the lesson to take away from this text is the great

possibilities that exist to sharpen our view of biology and of

medicine in particular. Future progress will involve refinement

of the methods described here. There are methods that will not

stand the test of time but taken as a whole, this book should

be widely read. Every chapter has extensive bibliographic references

and should serve as entrée into the literature even if the

specific examples are of only passing interest. The editors and

their coauthors are to be commended in providing a very thorough

and interesting sampling of the methods being developed

in the field."

Sid Aaron, Sid Aaron Consultants, LLC, in the International Journal of Toxicology, May 2006

From the Publisher