Teratogenicity Testing: Methods and Protocols

Teratogenicity Testing: Methods and Protocols

by Luís Félix (Editor)

Paperback(Softcover reprint of the original 1st ed. 2018)

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Overview

This detailed book provides a compilation of laboratory techniques and tests to assess the risks to embryo-fetal development from drug exposure during early developmental stages. After an introduction to teratogenicity testing, the contents explore both in vitro and in vivo techniques in the study of the teratogenic and fetotoxic effects of drugs, as well as numerous animal-based teratology methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and comprehensive, Teratogenicity Testing: Methods and Prools describes methods that integrate a battery of tests that can be performed in cells, organs, tissues, and animal models for evaluating toxicity and/or the safety of compounds in early developmental stages with the goal of estimating, preventing, or minimizing the teratogenic potential of drugs.

Product Details

ISBN-13: 9781493993147
Publisher: Springer New York
Publication date: 07/25/2019
Series: Methods in Molecular Biology , #1797
Edition description: Softcover reprint of the original 1st ed. 2018
Pages: 614
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

Part I: Introduction to Teratology Testing



1. An Overview of Teratology


Ana M. Calado and Maria dos Anjos Pires



2. Teratology Study Guidelines: An Overview


Sofia Alves-Pimenta, Lui's Félix, Bruno Colaço, Paula A. Oliveira, and Carlos Venâncio



3. Biological Concerns on the Selection of Animal Models for Teratogenic Testing


Sofia Alves-Pimenta, Bruno Colaço, Paula A. Oliveira, and Carlos Venâncio



Part II: In Vitro-Based Teratology Screening



4. The Validated Embryonic Stem Cell Test with Murine Embryonic Stem Cells


Lauren M. Walker, Darcie L.V. McClelland-Descalzo, and Nicole I. zur Nieden



5. Human Pluripotent Stem Cells to Assess Developmental Toxicity in the Osteogenic Lineage


Joseph V. Madrid, Steven R. Sera, Nicole R.L. Sparks, and Nicole I. zur Nieden



6. Chick Embryonic Cardiomyocytes Micromass System for Assessing Developmental Cardiotoxicity of Drugs


Wasay M.S. Qureshi and Margaret K. Pratten



7. Flow Cytometry to Evaluate Potential Developmental Toxicants in the Embryonic Stem Cell


Valéria Maria Lara, Kelly C. Santos Roballo, André Tadeu Gotardo, and Carlos Eduardo Ambrósio



8. Morphology-Based Whole Embryo Culture for Developmental Toxicity of Drugs


Bo Liang and Chi C. Wang



9. Western Blot Methodologies for Analysis of In Vitro Protein Expression Induced by Teratogenic Agents


Carlos Martins-Gomes and Amélia M. Silva



10. Manipulation of MicroRNAs in Cultured Mouse Embryos: Applications for Developmental Toxicology


Fuqiang Yuan and Shao-yu Chen



Part III: Animal-Based Teratology Methods



11. Insights into the Phenotypic and Behavioral Effects of Teratogenic Drugs in Caenorhabditis elegans


Caralina M. de Evsikova



12. Effect of Teratogens on Development of Drosophila melanogaster


Dong Li and Xiaolin Bi



13. Cellular Responses in Drosophila melanogaster Following Teratogen Exposure


Matheus C. Bianchini, José L.R. Portela, Robson L. Puntel, and Daiana S. Ávila



14. Behavioral Teratogenesis in Drosophila melanogaster


Monalisa Mishra and Bedanta Kumar Barik



15. Evaluation of the Teratogenicity of Pharmaceuticals Using FETAX


Hariz Islas-Flores, Itzayana Pérez-Alvaréz, and Leobardo M. Gómez-Oliván



16. Histological Observation of Teratogenic Phenotypes Induced in Frog Embryo Assays


Mark E. Pownall and Margaret S. Saha



17. Visualization of Gene Expression Patterns by In Situ Hybridization on Early Stages of Development of Xenopus laevis



Heithem M. El-Hodiri and Lisa E. Kelly



18. Analysis of Lethality and Malformations During Zebrafish (Danio rerio) Development


Azhwar Raghunath and Ekambaram Perumal



19. General Whole-Mount Immunohishemistry of Zebrafish (Danio rerio) Embryos and Larvae Prool


Dércia Santos, Sandra M. Monteiro, and Ana Luzio



20. Geometric Morphometrics as a Tool to Evaluate Teratogenic Effects in Zebrafish (Danio rerio)


Kathryn E. Perez and Tisha C. King-Heiden



21. Live Metabolic Profile Analysis of Zebrafish Embryos Using a Seahorse XF 24 Extracellular Flux Analyzer


Simon T. Bond, Kevin A. McEwen, Prusothman Yoganantharajah, and Yann Gibert



22. Behavioral Profiling of Zebrafish (Danio rerio) Larvae Following Teratogen Exposure


Ana M. Valentim



23. Omics in Zebrafish Teratogenesis


Benjamin Piña, Laia Navarro, Carlos Barata, Demetrio Raldúa, Rubén Martínez, and Marta Casado



24. Proteomic Analysis of Zebrafish (Danio rerio) after Chemical Exposure


Yew Mun Lee, Caixia Li, Siew Hong Lam, Zhiyuan Gong, and Qingsong Lin



25. Immunohishemical Assessment as a Tool for Investigating Developmental Toxicity in Zebrafish (Danio rerio)


Carla Santos and Maria de Lurdes Pinto



26. Oxidative Stress Assessment in Zebrafish Larvae


Inês Domingues and Carlos Gravato



27. Hemodynamic Studies for Analyzing the Teratogenic Effects of Drugs in the Zebrafish Embryo


Huseyin C. Yalcin



28. Western Blot Analysis and Immunostaining for Prediction of Embryotoxicity in Mus musculus


Yulia Irnidayanti, Win Darmanto, and Dias R. Sutiono



29. Histological and Hishemical Profile for Teratological Assessment in Mus musculus


Maria de Lurdes Pinto and Paula Rodrigues



30. In Vivo Analysis of Apoptosis in Embryonic Hippocampus


Ahmad Salimi and Jalal Pourahmad



31. Measurement of Mihondrial Toxicity Parameters in Embryonic Hippocampus



Ahmad Salimi and Jalal Pourahmad



32. Animal Tests for Evaluation of Cognitive Impairment in Neonatal Mouse


Ahmad Salimi and Jalal Pourahmad



33. Methodology of Genotoxic and Teratogenic Studies in Rats


Eyyup Rencuzogullari and Muhsin Aydin



34. Whole Mount In Situ Hybridization and Morphometric Analysis in Rabbit Embryos


Yoshinori Kawamura and Kiyoshi Matsumoto



Glossary of Key Terms for Teratology


Maria dos Anjos Pires and Ana M. Calado


Index

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