Two-Component Signaling Systems, Part A

by Melvin I. Simon (Editor), Brian Crane (Editor), Alexandrine Crane (Editor)

Overview

Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." Sections include: Computational Analyses of Sequences and Sequence Alignments Biochemical and Genetic Assays of Individual Components of Signaling Systems Physiological Assays and Readouts - Presents detailed protocols - Includes troubleshooting tips

Product Details

ISBN-13:	9780080548715
Publisher:	Elsevier Science & Technology Books
Publication date:	09/21/2011
Series:	Methods in Enzymology , #422
Sold by:	Barnes & Noble
Format:	eBook
Pages:	592
File size:	20 MB
Note:	This product may take a few minutes to download.

Table of Contents

Section I. Computational Analyses of Sequences and Sequence AlignmentsCHAPTER 1: Comparative genomic and protein sequence analyses of a complex system controlling bacterial chemotaxis.CHAPTER 2: Two component systems in microbial communities: Approaches and resources for analyzing metagenomic data sets.CHAPTER 3: Identification of sensory and signal-transducing domains in two-component signaling systems.CHAPTER 4: Features of protein-protein interactions in two-component signaling deduced from genomic libraries.CHAPTER 5: Sporulation phosphorelay proteins and their complexes: Crystallographic characterization.CHAPTER 6: Control Analysis of Bacterial Chemotaxis Signaling.CHAPTER 7: Classification of Response Regulators based on their Surface PropertiesSection II. Biochemical and Genetic Assays of Individual Components of the Signaling SystemsCHAPTER 8: Purification and Assays of Rhodobacter capsulatus RegB-RegA Two Component Signal Transduction System.CHAPTER 9: Purification and reconstitution of PYP-phytochrome (Ppr) with biliverdin and 4-hydroxycinnamic acid.CHAPTER 10: Oxygen and Redox-Sensing by Two-Component Systems That Regulate Behavioral Responses. Behavioral Assays and Structural Studies of Aer using in vivo Disulfide Crosslinking.CHAPTER 11: Two-Component Signaling in the Virulence of S. aureus: A Silkworm Larvae-Pathogenic Agent Infection Model of Virulence.CHAPTER 12: TonB System, In vivo Assays and CharacterizationCHAPTER 13: Biochemical Characterization of Plant Ethylene Receptors following Transgenic Expression in Yeast.CHAPTER 14: Structure of SixA, a histidine protein phosphatase of the ArcB HPt domain in E. coli.CHAPTER 15: Triggering and monitoring light-sensing reactions in protein crystalsCHAPTER 16CHAPTER 17: Application of Fluorescence Resonance Energy Transfer to Examine EnvZ/OmpR InteractionsCHAPTER 18: Gene promoter scan (GPS) methodology for identifying and classifying co-regulated promoters. CHAPTER 19: Targeting two-component signal transduction: A novel drug discovery system.CHAPTER 20: The essential YycFG two–component system of Bacillus subtilisSection III. Physiological Assays and ReadoutsCHAPTER 21: Isolation and Characterization of Chemotaxis Mutants of the Lyme Disease Spirochete Borrelia burgdorferi Using Allelic Exchange Mutagenesis, Flow Cytometry and Cell Tracking.CHAPTER 22: Phosphorylation assays of chemotaxis two-component system proteins in Borrelia burgdorferi. CHAPTER 23: Regulation of Respiratory Genes by ResD-ResE Signal Transduction System in Bacillus subtilis. CHAPTER 24: Physiological and genetic characterization of two-component systems in Myxococcus.CHAPTER 25: Detection and measurement of two-component systems that control dimorphism and virulence in fungi. CHAPTER 26: Using Two-Component Systems and other Bacterial Regulatory Factors for the Fabrication of Synthetic Genetic Devices.

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From the Publisher

Fueled by the explosion of sequence information provided by various genome projects, the number of two-component systems continues to rapidly grow.