Artificial DNA: Methods and Applications / Edition 1

by Yury E. Khudyakov, Howard A. Fields

Overview

Combining elements of biochemistry, molecular biology, and immunology, artificial DNA can be employed in a number of scientific disciplines. Some of the varied applications include site-specific mutagenesis, hybridization, amplification, protein engineering, anti-sense technology, DNA vaccines, protein vaccines, recombinant antibodies, screening for genetic and pathogenic diseases, development of materials with new biochemical and structural properties, and many more.

Artificial DNA: Methods and Applications introduces the concept of artificial DNA that has been rationally designed and explains how it may be exploited in order to develop products that will achieve your intended purpose. The first part of the book covers methods of oligonucleotide synthesis and direct applications of synthetic DNA. The second part describes methods of gene assembly from synthetic oligonucleotides and applications of synthetic genes. The authors also discuss the different trends and future developments within each application area .

With state-of-the art research, the contributing authors describe how to engineer proteins using rational and semi-rational design to exhibit the desired traits and detail the various amplification reactions and hybridization techniques for modeling evolution and for use in basic research. The only text devoted to this subject, Artificial DNA offers a comprehensive review that allows you to understand the strategy, design, and applications of synthetic oligonucleotides.

Product Details

ISBN-13:	9780367395810
Publisher:	Taylor & Francis
Publication date:	09/05/2019
Pages:	440
Product dimensions:	7.00(w) x 10.00(h) x (d)

About the Author

Yury E. Khudyakov, Howard A. Fields

Table of Contents

Chapter 1 Chemical Synthesis of Oligonucleotides: From Dream to Automation Richard T. Pon 1

Chapter 2 Artificial Hybridization Probes Salvatore A. E. Marras 71

Chapter 3 Peptide Nucleic Acid (PNA): A DNA Mimic with a Pseudopeptide Backbone Frederik Beck Peter E. Nielsen 91

Chapter 4 Synthetic DNA Used in Amplification Reactions Lisa S. Kelly 115

Chapter 5 Artificial Nucleic Acids and Genome Profiling Lilia M. Ganova-Raeva 161

Chapter 6 Site-Directed Mutagenesis John J. Glass Beverly A. Heinz 199

Chapter 7 Mimotopes: An Overview Renu Tuteja 225

Chapter 8 Artificial Genes for Chimeric Virus-Like Particles Paul Pumpens Elmars Grens 249

Chapter 9 DNA Vaccines Sang Won Han Jane Zveiter de Moraes Célio Lopes Silva Roger Chammas Mauricio Martins Rodrígues 329

Chapter 10 Toward Designer Diagnostic Antigens Joy Chih-Wei Chang 363

Chapter 11 Recombinant Antibodies Ramón Montaño Flor H. Pujol 385

Index 411