Protocols for Neural Cell Culture

by Sergey Fedoroff (Editor), Arleen Richardson (Editor)

Overview

The first edition of Protocols for Neural Cell Culture was published in 1992 and the second edition in 1997. Originally, the publication grew outofprotocols used in the Tissue Culture Course given at the University of Saskatchewan. The course was patterned on those given by the Tissue CultureAssociation, first in Toronto, Canada, in 1948, then in Cooperstown, NY, then Denver, CO, and finally in Madison, WI, where the course ended in 1964. The course in Saskatchewan began in 1963 as a month-long international course that included both animal and plant tissue cultures. Over the years the course underwent specialization, first being limited to animal tissue culture, then to an intensive one-week general course. This led to one-week courses especially designed for tissue culture for the study of cancer or of the cardiovascular or the nervous system. In 1989, the Saskatchewan course became part of the Tissue Culture Training Facility of the Neuroscience Network of the Canadian Network of Centres of Excellence. The course and the Training Facility ceased to exist in 1997. The faculty for the Saskatchewan course was drawn from the best labora­ tories in the world and laboratory protocols from those centers were thoroughly tested in a student laboratory setting for many years.
     

Product Details

ISBN-13:	9781592592074
Publisher:	Springer-Verlag New York, LLC
Publication date:	06/29/2008
Series:	Springer Protocols Handbooks
Sold by:	Barnes & Noble
Format:	eBook
Pages:	384
File size:	7 MB

Table of Contents

In Vitro Assays for Axonal Growth and Targeting.- Interface Organotypic Hippocampal Slice Cultures.- Slice Cultures for Study of Microglia.- Microexplant Cultures of the Cerebellum.- Construction and Use of Compartmented Cultures for Studies of Cell Biology of Neurons.- Chick Spinal Somatic Motoneurons in Culture.- Primary Cultures of Sympathetic Ganglia.- Primary Cell Cultures for the Study of Myelination.- Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures.- Culture of Glial Cells from Human Brain Biopsies.- Cultures of Astroglia and Microglia from Primary Cultures of Mouse Neopallium.- Cultures of Oligodendroblasts from Primary Cultures of Rat Neopallium.- Olfactory Ensheathing Cell Cultures.- Cultures of Stem Cells of the Central Nervous System.- Aggregating Neural Cell Cultures.- Neural Cell Lines.- Virus Vectors for Gene Therapy of the Nervous System.- Preparation of Substrata for In Vitro Culture of Neurons.- Serum-Free Media forNeural Cell Cultures.- Development of Multiple-Well Plate Biological Assays.- Neural Cell Counting.- Assay for Neuronal Cell Migration.- Colony Cultures.- Hybridoma Cultures for Production of Antibodies.- Elimination of Cell Types from Mixed Neural Cell Cultures.- Quantification of Cells in Culture.- Tips for Tissue Culture.

Editorial Reviews

The first 12 chapters in this manual cover slice cultures, explants, and primary cell cultures from animals that are widely used in neuroscience, as well as human biopsies. Eight additional chapters discuss various methods of analysis, from general tips on recommended equipment and routine techniques to sophisticated microscopy and gene therapy experiments using herpesvirus. The editors have achieved consistent format of chapters with very moderate overlapping. The purpose of the book is to provide a practical manual for experimentation in neuroscience. The new edition is updated by several new chapters. It may serve as a benchtop laboratory manual and reference. In addition, it offers a fresh look at hot topics of neuroscience. A good example is the a brief discussion of the presence of astroglial precursors in brain cultures (chapter 11). The potential audience for the book ranges from a principal investigator of a project to a part-time undergraduate working in a neuroscience lab. Anyone interested in neuroscience can find descriptions of basic experimental facts and their immediate interpretation. The book is clearly written and gives all the necessary experimental details. It has a list of abbreviations and detailed subject index. Lists of references contain mostly appropriate books and reviews. Black-and-white photographs are of high quality and very informative. They include microphotographs and schemes and sometimes illustrate the organization of the workplace. Most chapters present an outline of the possible goals of specific experiments. The authors wisely refrain from detailed discussions of issues; instead, they lay strong laboratory background for a wide varietyof studies. This approach makes the book a pleasure to read, besides its immediate value as a reference source for routine work. The book is indispensable for every project in neuroscience.

Alexander Kolchinsky

This second edition of a collection of reproducible methods includes key techniques to study the development and differentiation of the nervous system, new methods for isolating and propagating progenitor cells, and timely procedures for eliminating contaminating neural or nonneural cells. New procedures for the transfection of cells in culture with nontoxic mutant herpes simplex virus are included, as are procedures for quantification, growth assays, evaluating neuroactive agents, preparing neural material for electron microscope analysis, and dissection. Plastic comb binding. Annotation c. by Book News, Inc., Portland, Or.

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