Recombinant DNA Principles and Methodologies

by James Greene (Editor)

Overview

This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications.
Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies!

Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as

plasmid biology

nucleic acid biochemistry

restriction enzymes

cloning strategies

gel electrophoresis

southern and northern blotting

preparation of probes

phage lambda biology

cosmids and genome analysis

cloned gene expression

polymerase chain reaction

conventional and automated DNA sequencing

site-directed mutagenesis

and more!
Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.

Product Details

ISBN-13:	9781000148688
Publisher:	CRC Press
Publication date:	12/16/2021
Sold by:	Barnes & Noble
Format:	eBook
Pages:	768
File size:	8 MB

About the Author

James Greene (Catholic University, Washington D.C., USA)

Table of Contents

Why Cloning?, James J. Greene Biochemistry of Nucleic Acids, Paul S. Miller Enzymology of Recombinant DNA, Frank J. Castora Basic Escherichia coli Genetics Useful for Recombinant DNA Techniques, Darren D. Sledjeski and Susan Garges Formulation of Cloning Strategies, John B. Dame Isolation and Purification of Nucleic Acids, James J. Greene Gel Electrophoresis, Southern and Northern Analysis, James J. Greene and Frank J. Castora Hybridization Probes, Frank J. Castora and James J. Greene cDNA Cloning and the Construction of Recombinant DNA, Dante S. Zarlenga Molecular Cloning by Plasmid Vectors, Paulina Balbás and Francisco Bolívar Phage Lambda: Basic Biology and Use as a Vector, Susan Frackman and Michael Feiss Cosmid Cloning, John Collins and Werner Lindenmaier Multipurpose Cloning Vectors, August J. Sick, Joseph M. Fernandez, and Jay M. Short Mammalian Expression Vectors, Ricky R. Hirschhorn and Nava Sarver Approaches to the Expression of Foreign Genes, Allan R. Shatzman Screening for Specific Recombinant Clones, Lucia Irene González-Villaseñor and Mark M. Manak Principles and Applications of the Polymerase Chain Reaction, Garbriela S. Dveksler and Carl W. Dieffenbach DNA Sequencing, Maurice A. Kashdan, Phillip T. Moen, Jr.,Venigalla B. Rao, and Bachtoi S. Rao Site-Directed Mutagenesis, Alfred Johnson and Marvin Reitz

Editorial Reviews

"

…provide[s] clear insights into the biological, chemical, and physical rationale behind common molecular methods used in lab manuals and commericial kits.

"- Trends in Genetics

From the Publisher

...provide[s] clear insights into the biological, chemical, and physical rationale behind common molecular methods used in lab manuals and commericial kits.

Trends in Genetics

Nineteen contributions emphasize the principles and rationale underlying recombinant DNA methodology while providing a general understanding of experimental protocols. Basic and advanced topics include plasmid biology, nucleic acid biochemistry, restriction enzymes, cloning strategies, gel electrophoresis, southern and northern blotting, preparation of probes, phage lambda biology, cosmids and genome analysis, cloned gene expression, polymerase chain reaction, conventional and automated DNA sequencing, and site-directed mutagenesis. Annotation c. by Book News, Inc., Portland, Or.

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