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This volume presents a list of cutting-edge prools for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Prools guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard prools of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to...