Capillary Electrophoresis Guidebook: Principles, Operation, and Applications / Edition 1

by Kevin D. Altria

Overview

This book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap­ idly maturing technique, detailed validated prools are not widely established. Therefore, extensive experimental procedures are not provided for individual analyses. The intention is to provide general guide­ lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for activities such as changing capillaries, method development, quantitative procedures, optimization of precision and sensitivity, and the validation of methods, fraction collection, and troubleshooting, as well as a quick guide to running a separation. The application range of CE is possibly the most diverse of all analytical techniques and ranges from large, complex macromolecules, such as proteins and nucleic acids, to small solutes, such as organic drugs and inorganic anions and cations.

Product Details

ISBN-13:	9781617370113
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/10/2010
Series:	Methods in Molecular Biology , #52
Edition description:	Softcover reprint of hardcover 1st ed. 1996
Pages:	349
Product dimensions:	5.98(w) x 9.02(h) x 0.03(d)

Table of Contents

General Guidelines to the Operation of Capillary Electrophoresis Methods and Instrumentation.- Fundamentals of Capillary Electrophoresis Theory.- Standard Commercial Instrument Description.- Typical Operating Procedures.- Method Development/Optimization.- Quantitation Procedures.- Optimization of Precision in Quantitative Analysis.- Optimization of Sensitivity.- Method Validation.- Fraction Collection.- Troubleshooting.- Quick Guide to Running a Successful Separation.- Applications of Capillary Electrophoresis and Specific Technologies.- Micellar Electrokinetic Chromatography.- Capillary Gel Electrophoresis.- Chiral Separations by Capillary Electrophoresis.- Capillary Electrochromatography.- Application and Limits of Sample Stacking in Capillary Electrophoresis.- Analysis of Bases, Nucleosides, and (Oligo)nucleotides by Capillary Electrophoresis.- Application of Capillary Electrophoresis to Pharmaceutical Analysis.- Separation of Peptides and Protein Digests by Capillary Electrophoresis.- Additional Application Areas of Capillary Electrophoresis.

Editorial Reviews

The book is a very useful guidebook for CE practitioners as well as a reference book for researchers and graduate students. I highly recommend this book for libraries and individuals active in the field of CE.-FEBS Letters

From the Publisher

Reviewer: Earle W. Holmes, PhD (Loyola University Medical Center)
Description: This book presents the general theory and practice of capillary electrophoresis (CE) and also contains nine chapters devoted to specific techniques and applications. The latter include topics such as micellar electrokinetic chromatography, capillary gel electrophoresis, chiral separations, analysis of bases, nucleosides and oligonucleotides, and separation of peptides and protein digests.
Purpose: The book is intended as a working guide to the operation of CE instrumentation and as a review of some of the important applications of this relatively new technique.
Audience: The book would be of interest to researchers in academia and industry who wish to apply CE to their work.
Features: The material presented was kept as current as possible by including "notes added in proof at the end of each chapter." Here, the most recent findings relevant to the subject are presented, and references to newly published work are cited.
Assessment: This text offers a competent overview of CE; however, it is not as comprehensive as some other CE texts that have recently appeared. The actual quantity of information presented is less than the text's 349 pages would suggest. The scope of applications presented is rather limited, and many of these are not of direct interest to molecular biologists. The book does not contain enough novel features to recommended it over previously published texts on this topic. Libraries who are determined to collect the complete series might wish to purchase this volume.

Doody's Review Service