Cell Culture and Upstream Processing

Cell Culture and Upstream Processing

by Michael Butler

NOOK Book(eBook)

$135.99 $155.00 Save 12% Current price is $135.99, Original price is $155. You Save 12%.
View All Available Formats & Editions

Available on Compatible NOOK Devices and the free NOOK Apps.
WANT A NOOK?  Explore Now


Upstream processing refers to the production of proteins by cells genetically engineered to contain the human gene which will express the protein of interest. The demand for large quantities of specific proteins is increasing the pressure to boost cell culture productivity, and optimizing bioreactor output has become a primary concern for most pharmaceutical companies.  Each chapter in Cell Culture and Upstream Processing is taken from presentations at the highly acclaimed IBC conferences as well as meetings of the European Society for Animal Cell Technology (ESACT) and Protein Expression in Animal Cells (PEACe) and describes how to improve yield and optimize the cell culture production process for biopharmaceuticals, by focusing on safety, quality, economics and operability and productivity issues.

Cell Culture and Upstream Processing will appeal to a wide scientific audience, both professional practitioners of animal cell technology as well as students of biochemical engineering or biotechnology in graduate or high level undergraduate courses at university.

Product Details

ISBN-13: 9781134148295
Publisher: CRC Press
Publication date: 08/07/2007
Sold by: Barnes & Noble
Format: NOOK Book
Pages: 250
File size: 2 MB

About the Author

Michael Butler-Associate Dean of Science; Professor of Cell Technology, Department of Microbiology, School of Science, University of Manitoba, Winnipeg, Canada

Table of Contents

1. Cell Line Development and Culture Strategies: Future Prospects to Improve Yields  2. Use of DNA Insulator Elements and Scaffold / Matrix-Attached Regions for Enhanced Recombinant Protein Expression  3. Targeted Gene Insertion to Enhance Protein Production From Cell Lines  4. Recombinant Human IgG Production From Myeloma and Chinese Hamster Ovary Cells  5. Cell Culture Media Development: Customization of Animal Origin-Free Components and Supplements  6. Post-Translational Modification of Recombinant Proteins  7. Metabolic Engineering to Control Glycosylation  8. An Alternative Approach: Humanization of N-glycosylation Pathways in Yeast  9. Perfusion or Fed-Batch? A Matter of Perspective

Customer Reviews

Most Helpful Customer Reviews

See All Customer Reviews