Globins and Other Nitric Oxide-Reactive Proteins, Part B

Globins and Other Nitric Oxide-Reactive Proteins, Part B

by Robert K. Poole

NOOK Book(eBook)

View All Available Formats & Editions

Available on Compatible NOOK Devices and the free NOOK Apps.
WANT A NOOK?  Explore Now


The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences.

Methods in Enzymology is now available online at ScienceDirect — full-text online of volumes 1 onwards. For more information about the Elsevier Book Series on ScienceDirect Program, please visit:

This volume is the second of two planned volumes on the topic of globin and other nitric oxide-reactive proteins.

Product Details

ISBN-13: 9780080877921
Publisher: Elsevier Science
Publication date: 09/02/2011
Series: Methods in Enzymology , #437
Sold by: Barnes & Noble
Format: NOOK Book
Pages: 712
File size: 32 MB
Note: This product may take a few minutes to download.

About the Author

Professor Robert Poole is West Riding Professor of Microbiology at the University of Sheffield. He has >35 years’ experience of bacterial physiology and bioenergetics, in particular O2-, CO- and NO-reactive proteins, and has published >300 papers (h=48, 2013). He was Chairman of the Plant and Microbial Sciences Committee of the UK Biotechnology and Biological Sciences Research Council and has held numerous grants from BBSRC, the Wellcome and Leverhulme Trusts and the EC. He coordinates an international SysMO systems biology consortium. He published pioneering studies of bacterial oxidases and globins and discovered the bacterial flavohaemoglobin gene (hmp) and its function in NO detoxification He recently published the first systems analyses of responses of bacteria to novel carbon monoxide-releasing molecules (CORMs) and is a world leader in NO, CO and CORM research.

Table of Contents

Section I. Nitric Oxide-Metabolising and Detoxifying Enzymes

Chapter 1
Structural Studies on Flavodiiron Proteins
João B. Vicente, Maria Arménia Carrondo, Miguel Teixeira and Carlos Frazão

Chapter 2
Biochemical, Spectroscopic and Thermodynamic Properties of Flavodiiron Proteins
João B. Vicente, Marta C. Justino, Vera L. Gonçalves, Lígia M. Saraiva and Miguel Teixeira

Chapter 3
Kinetic Characterization of the Escherichia coli Nitric Oxide Reductase Flavorubredoxin
João B. Vicente, Francesca M. Scandurra, Elena Forte, Maurizio Brunori, Paolo Sarti, Miguel Teixeira and Alessandro Giuffrè

Chapter 4
Escherichia coli cytochrome c-nitrite reductase NrfA
Thomas A. Clarke, Paul C. Mills, Susie R. Poock, Julea N. Butt1, Myles R. Cheesman, Jeffrey A. Cole, Jay C. D. Hinton, Andrew M. Hemmings, Gemma Kemp, Christopher Söderberg, Stephen Spiro, Jessica Van Wonderen, David J. Richardson

Chapter 5
The respiratory NO reductase (NorBC) from Paracoccus denitrificans
Sarah J. Field, Faye H. Thorndycroft, Andrey D. Matorin,
David J. Richardson and Nicholas J. Watmough

Chapter 6
Redox-controlled dinitrosyl formation at the diiron-oxo center of NorA
Rainer Cramm, Katja Strube

Chapter 7
Purification and functional analysis of fungal nitric oxide reductase cytochrome P450nor
Li Zhangand Hirofumi Shoun

Chapter 8
A Quantitative approach to nitric oxide inhibition of terminal oxidases of the respiratory chain
Maria G. Mason, Rebecca S. Holladay, Peter Nicholls, Mark Shepherd and Chris E. Cooper

Section II. Sensor Proteins
Chapter 9
Cloning, Expression, and Purification of the N-terminal Heme Binding Domain of the Globin-Coupled Sensors
Jennifer A. Saito, Tracey Allen K. Freitas, and Maqsudul Alam

Chapter 10
Oxygen-Sensing Histidine-Protein Kinases: Assays of Ligand-Binding and Turnover of Response-Regulator Substrates
Marie-Alda Gilles-Gonzalez, Gonzalo Gonzalez, Eduardo H. S. Sousa, and Jason Tuckerman

Chapter 11
Reactions of nitric oxide and oxygen with FNR (regulator of Fumarate and Nitrate Reduction), a global transcriptional regulator, during anaerobic growth of Escherichia coli
Jason C. Crack, Nick E. Le Brun, Andrew J. Thomson, Jeffrey Green and Adrian J. Jervis

Chapter 12
Genome wide identification of binding sites for the nitric oxide sensitive transcriptional regulator NsrR.
Sam Efromovich, David Grainger, Diane Bodenmiller, and Stephen Spiro.

Chapter 13
Methods in Enzymology: Globins and other NO-reactive proteins. Characterization of the NO-reactive transcriptional activator NorR.
Benoît D’Autréaux, Nick Tucker, Stephen Spiro and Ray Dixon

Section III. Advanced Spectroscopic Methods

Chapter 14
Hemoglobins from Mycobacterium tuberculosis and Campylobacter jejuni: A Comparative Study with Resonance Raman Spectroscopy
Changyuan Lu, Tsuyoshi Egawa, Masahiro Mukai, Robert K. Pooleand Syun-Ru Yeh

Chapter 15
The power of using continuous-wave and pulsed electron paramagnetic resonance methods for the structure analysis of the ferric forms and NO-ligated ferrous forms of globins.
Sabine Van Doorslaer and Filip Desmet

Chapter 16
Oxygen binding to haem proteins in solution, encapsulated in silica gels and in the crystalline state
Luca Ronda, Stefano Bruno, Serena Faggiano, Stefano Bettati and Andrea Mozzarelli

Chapter 17
Characterization of ligand migration mechanisms inside haemoglobins from the analysis of geminate rebinding kinetics
Stefania Abbruzzetti, Stefano Bruno, Serena Faggiano, Luca Ronda, Elena Grandi, Andrea Mozzarelli, Cristiano Viappiani

Chapter 18
Ligand Dynamics in Heme Proteins Observed by
Fourier Transform Infrared Spectroscopy at Cryogenic Temperatures
Karin Nienhaus, G. Ulrich Nienhaus

Chapter 19
Time-resolved x-ray crystallography of heme proteins
Vukica Šrajer and William E. Royer, Jr

Chapter 20
Structural Dynamics of Myoglobin
Maurizio Brunori, Dominque Bourgeois and Beatrice Vallone

Chapter 21
Use of the Conjugate Peak Refinement Algorithm for Identification of Ligand Binding Pathways in Globins
Stephen D. Golden and Kenneth W. Olsen

Chapter 22
Finding gas migration pathways in proteins using implicit ligand sampling
Jordi Cohen, Kenneth W Olsen, and Klaus Schulten

Chapter 23
Identification of Ligand Binding Pathways in Truncated Hemoglobins Using Locally Enhanced Sampling Molecular Dynamics
Stephen D. Golden and Kenneth W. Olsen

Customer Reviews

Most Helpful Customer Reviews

See All Customer Reviews