Methods for Monitoring Mitochondrial Functions During Pathogen Infection

by Saverio Marchi (Editor), Lorenzo Galluzzi (Editor)

Overview

Methods for Monitoring Mitochondrial Functions During Pathogen Infection, Volume 194 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Mitochondrial Labeling with Fluorescent Proteins for Enhanced Morphological Characterization and Visualization of Intercellular Mitochondrial Transfer, Visualizing mitochondrial electron transport chain complexes and super-complexes during infection of human macrophages with Legionella pneumophila, Quantitative assessment of mitochondrial membrane potential in macrophages in sepsis, Quantification of mitochondrial reactive oxygen species in macrophages during sepsis, and much more.Additional chapters cover Quantification of intracellular and mitochondrial ATP content in macrophages during lipopolysaccharide-induced inflammatory response, Analysis of cytosolic mtDNA release during Staphylococcus Aureus infection, Monitoring mitochondria dynamics upon infection using a tomographic approach, A screening system to determine bacterial effects on MADCAM1 expression by high endothelial venule cells, Isolation of mitochondria from Mycobacteria infected cells, Monitoring mitochondrial localization of Mycobacterial proteins, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Cell Biology series - Includes the latest information on the topic of development, characterization, and applications in CAR T Cells

Product Details

ISBN-13:	9780323992206
Publisher:	Elsevier Science & Technology Books
Publication date:	03/10/2025
Series:	Methods in Cell Biology , #194
Sold by:	Barnes & Noble
Format:	eBook
Pages:	12
File size:	20 MB
Note:	This product may take a few minutes to download.

About the Author

Saverio Marchi obtained his PhD in ‘Pharmacology and Molecular Oncology’ from University of Ferrara (Italy), under the supervision of Prof. Rosario Rizzuto, where he investigated the role of Ca2+ signalling and mitochondrial dynamics in cancer. He did a short term research stay at the Gustave Roussy Institute (Paris, France) in the Guido Kroemer’s lab, for the study of the mechanisms of autophagy regulation. On 2011 he joined the Laboratory of Dr. Paolo Pinton at the University of Ferrara (Italy) where he carried out his postdoctoral studies. During his postdoctoral training, he studied the implications of Endoplasmic Reticulum-mitochondria connection in diseases, especially cancer. In 2014, he was the recipient of the Young Researcher award, which allowed him to work at the Cancer Center, University of Hawai’i (Prof. Michele Carbone’s lab), where he was introduced to the study of the signalling pathways regulating mesothelioma progression. Now, he is associate professor at the Marche Polytechnic University, where he leads his own group. He is interested in the involvement of the MCU complex and mitochondrial Ca2+ in the evolution of cancer. More recently, he has become interested in the alteration of mitochondrial dynamics during bacterial infection.
He published 77 papers on “peer-reviewed” journals (h-index: 41; n° of citations > 5800. Source: Scopus).

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.

Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Table of Contents

1. Mitochondrial Labeling with Fluorescent Proteins for Enhanced Morphological Characterization and Visualization of Intercellular Mitochondrial TransferToshio Miki2. Visualizing mitochondrial electron transport chain complexes and super-complexes during infection of human macrophages with Legionella pneumophilaPedro Escoll3. Quantitative assessment of mitochondrial membrane potential in macrophages in sepsisGuochang Hu4. Quantification of mitochondrial reactive oxygen species in macrophages during sepsisGuochang Hu5. Quantification of intracellular and mitochondrial ATP content in macrophages during lipopolysaccharide-induced inflammatory responseGuochang Hu6. Analysis of cytosolic mtDNA release during Staphylococcus Aureus infectionSaverio Marchi7. Monitoring mitochondria dynamics upon infection using a tomographic approachGianluca Morroni8. A screening system to determine bacterial effects on MADCAM1 expression by high endothelial venule cellsOliver Kepp9. Isolation of mitochondria from Mycobacteria infected cellsKrishnaveni Mohareer10. Monitoring mitochondrial localization of Mycobacterial proteinsKrishnaveni Mohareer11. Bacterial predators and BALOs: growth protocol and relation with mithochondriaValerio Iebba12. Assessment of adhering and invading properties of Escherichia coli strainsValerio Iebba13. Bioinformatic techniques to study intracellular bacteriaValerio Iebba

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From the Publisher

Edited by one of the best scientists in the field, this series presents the latest updates in the field of cell biology