R-Loops: Methods and Protocols

This detailed book compiles a series of laboratory prools covering the most important aspects of R—loop biology. Beginning with a range of methods allowing for the detection of DNA—RNA hybrids, as well as their purification and visualization by electron microscopy, the volume continues with methods based on the use of RNase H—derived tools to detect DNA—RNA hybrids in vitro and in vivo. Several prools permit studying non—canonical RNA nucleotides in the R—loop context, as well as a number o...