Regulatory Non-Coding RNAs: Methods and Protocols

by Gordon G. Carmichael

Overview

Regulatory Non-Coding RNAs: Methods and Prools offers a collection of methods for those interested in the discovery, localization, and functional analysis of these non-coding transcripts that have the potential and ability to orchestrate and control gene expression. After a review of the field, this detailed volume continues with methods useful for the study of siRNAs, microRNAs and their targets, techniques concerned with long non-coding RNAs, as well as studies of the critical parameters of functional non-coding RNA protein-RNA interactions and the environment in which they act. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools and tips for troubleshooting and avoiding known pitfalls.

Dependable and easy to use, Regulatory Non-Coding RNAs: Methods and Prools provides a current, state-of-the-art collection of methods and approaches that will be of value to researchers in this expanding and fascinating field.

Product Details

ISBN-13:	9781493941841
Publisher:	Springer New York
Publication date:	09/22/2016
Series:	Methods in Molecular Biology , #1206
Edition description:	Softcover reprint of the original 1st ed. 2015
Pages:	181
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Diverse Functions and Mechanisms of Mammalian Long Non-Coding RNAs.- Sequence-Defined Oligoaminoamides for the Delivery of siRNAs.- A Biochemical Approach to Identify Direct MicroRNA Targets.- Native Gel Analysis for Mammalian MicroRNPs Assembled from Pre-MicroRNAs.- Identification and Validation of miRNA Target Sites within Non-Traditional miRNA Targets.- Fractionation of Non-Polyadenylated and Ribosomal-Free RNAs from Mammalian Cells.- Affinity-Purification of Long Noncoding RNA−Protein Complexes from Formaldehyde-Cross linked Mammalian Cells.- RNA Pull down Prool for In Vitro Detection and Identification of RNA-Associated Proteins.- nRIP-seq: A Technique to Identify RNA Targets of an RNA Binding Protein on a Genome-Wide Scale.- Analysis of the Subcellular Distribution of RNA by Fluorescence In Situ Hybridization.- RNA Fluorescence In Situ Hybridization in Cultured Mammalian Cells.- Locating RNAs In Situ with FISH-STIC Probes.- Rapid and Efficient Elimination of Specific Nuclear Noncoding RNAs in Mammalian Cells with Antisense Oligonucleotides.- On Using Magnesium and Potassium Ions in RNA Experiments.- Studying the Affinity, Kinetic Stability and Specificity of RNA/Protein Interactions: SINE ncRNA/Pol II Complexes as a Model System.