-There is an acceleration in prion disease research because of the spread of mad cow disease.
-This book covers in vitro, cellular, and animal models adapted for the study of TSEs.
-Includes bio-saftey procedures.
Table of ContentsFrom the contents: Abbreviations.- Transmissible Spongiform Encephalopathies or Prion Diseases (General Introduction).- Purification of PrPC.- Purification of the Pathological Isoform of Prion Protein (PrPsc or PrP-res) from Transmissible Spongiform Encephalopathy-affected Brain Tissue. Rapid Procedure for Purification and Renaturation of Recombinant PrP Protein.- Animal Models of Transmissible Spongiform Encephalopathies: Experimental Infection, Observation and Tissue Collection.- Cell Culture Models of TSEs. PrPsc Immunohistochemistry.- Western Immunoblotting Techniques for the Study of Transmissible Spongiform Encephalopathies.- Antibody Production and ELISA, Diagnosis of TSEs, General Considerations.- TSE Strain Typing in Mice.- Biosafety and Decontamination Procedures.- Cell-free Conversion of Prion Proteins.- Cytotoxicity of PrP Peptides.- Cyclic Amplification of Prion Protein Misfolding. Guide to Solutions.- Guide to Protocols.- Index