Techniques in Prion Research

Techniques in Prion Research

Paperback(Softcover reprint of the original 1st ed. 2004)

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Product Details

ISBN-13: 9783764322243
Publisher: Birkhäuser Basel
Publication date: 03/24/2005
Series: Methods and Tools in Biosciences and Medicine
Edition description: Softcover reprint of the original 1st ed. 2004
Pages: 221
Product dimensions: 7.01(w) x 10.00(h) x 0.02(d)

Table of Contents

From the contents: Abbreviations.- Transmissible Spongiform Encephalopathies or Prion Diseases (General Introduction).- Purification of PrPC.- Purification of the Pathological Isoform of Prion Protein (PrPsc or PrP-res) from Transmissible Spongiform Encephalopathy-affected Brain Tissue. Rapid Procedure for Purification and Renaturation of Recombinant PrP Protein.- Animal Models of Transmissible Spongiform Encephalopathies: Experimental Infection, Observation and Tissue Collection.- Cell Culture Models of TSEs. PrPsc Immunohistochemistry.- Western Immunoblotting Techniques for the Study of Transmissible Spongiform Encephalopathies.- Antibody Production and ELISA, Diagnosis of TSEs, General Considerations.- TSE Strain Typing in Mice.- Biosafety and Decontamination Procedures.- Cell-free Conversion of Prion Proteins.- Cytotoxicity of PrP Peptides.- Cyclic Amplification of Prion Protein Misfolding. Guide to Solutions.- Guide to Protocols.- Index

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