The Nucleus

This volume presents detailed, recently—developed prools ranging from isolation of nuclei to purification of chromatin regions containing single genes, with a particular focus on some less well—explored aspects of the nucleus. The methods described include new strategies for isolation of nuclei, for purification of cell type—specific nuclei from a mixture, and for rapid isolation and fractionation of nucleoli. For gene delivery into and expression in nuclei, a novel gentle approach using go...