The Ubiquitin Proteasome System: Methods and Protocols

“This volume explores numerous techniques used to study the ubiquitin proteasome system. The chapters in this book are organized into five parts and cover topics such as determining the mechanisms of action for E2s, E3s, and DUB enzymes; the latest advances to study the formation of poly—ubiquitin chains as well as their linkage types; the binding partners of proteins in the UPS; methods for structure determination by x—ray crystallography, cryo electron microscopy and SAXS; screening assay...