Supercritical Fluid Methods and Protocols / Edition 1

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Experienced practitioners present detailed accounts of a wide variety of techniques using supercritical fluids. These range from the supercr itical fluid extraction methods for numerous compounds to the ninhydri n staining of fingerprints on checks and banknotes, and from the detec tion of impurities in pharmaceuticals to a wide variety of application s throughout environmental and food science, and across analytical, cl inical, and medicinal chemistry. Detailed step-by-step instructions en able users to apply these essential techniques successfully the first time and include modifications that permit their effective adaptation to novel experimental or process conditions. Versatile and comprehensi ve, Supercritical Fluid Methods and Protocols offers both novice and e xperienced investigators and laboratory analysts powerful tools that e nable successful biological and bioprocess analyses and optimization t oday.

This book provides a detailed instruction manual for those wanting to use supercritical fluid techniques for the first time. Each chapter has been written by experienced researchers and they will enable workers with some or no previous experience of supercritical fluids to conduct experiments successfully at the first attempt. Detailed, step-by-step instructions are given to avoid any possibility of error, along with invaluable hints and tips about pitfalls to avoid. Extensive procedural notes explain why certain steps are necessary, how to optimize a method in a particular system, and offer alternative ways of modifying a technique for application to new situations.

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Editorial Reviews

From the Publisher
"Supercritical Fluid is a worthwhile addition to the reference collection of an academic or a special library that serves scientists who carry out extractions or perform liquid chromatography. It will be of special interest to researchers who are seeking solutions to reduce the amount of organic solvent waste material and its related pollution in their laboratories and on their campuses."—Review from E-Streams Vol.3, No. 10, Dec. 2000.

"The book is unique in the supercritical realm in that it is basically a "laboratory manual" and/or a compendium of a wide variety of applications that employ supercritical fluids....The strongest point of the book is the wide diversity in the applications that are demonstrated. This allows the reader no only to see who the investigators are that deal in supercritical fluids but also to ascertain how the experimental strategies differ depending upon the application....This will be an excellent book for anyone working with supercritical fluids and those looking for new applications....should be useful in affording the proper experimental direction."-Journal of Natural Products

"...the editors have drawn on their extensive academic and industrial experience to provide a source book of information on the application of supercritical fluids....The emphasis is on instructions, detailed enough for the neophyte, but with enough discussion to be of value to the more established practitioner....The wide range, clear presentation, uniformity of style and sheer usefulness of this book should guarantee it a place on the shelf of every biotechnologist." - Chromatographia

Experienced practitioners present detailed accounts of numerous techniques using supercritical fluids. These range from the supercritical fluid extraction methods for numerous compounds to the staining of fingerprints on checks and banknotes<-->and draw from analytical, clinical, and medical chemistry. Detailed step-by-step instructions enable users to apply these essential techniques successfully and include modifications that permit their effective adaptation to novel experimental of process conditions. Annotation c. Book News, Inc., Portland, OR (
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Product Details

  • ISBN-13: 9781617370793
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/19/2010
  • Series: Methods in Biotechnology Series , #13
  • Edition description: Softcover reprint of hardcover 1st ed. 2000
  • Edition number: 1
  • Pages: 256
  • Product dimensions: 0.61 (w) x 6.00 (h) x 9.00 (d)

Table of Contents

Introduction to Supercritical Fluids and Their Applications, Anthony A. Clifford and John R. Williams. Supercritical Fluid Extraction of Caffeine from Instant Coffee, John R. Dean, Ben Liu, and Edwin Ludkin. Supercritical Fluid Extraction of Nitrosamines from Cured Meats, John W. Pensabene and Walter Fiddler. Supercritical Fluid Extraction of Melengestrol Acetate from Bovine Fat Tissue, Robert J. Maxwell, Owen W. Parks, Roxanne J. Shadwell, Alan R. Lightfield, Carolyn Henry, and Brenda S. Fuerst. Supercritical Fluid Extraction of Polychlorinated Biphenyls from Fish Tissue, Michael O. Gaylor and Robert C. Hale. Isolation of Polynuclear Aromatic Hydrocarbons from Fish Products by Supercritical Fluid Extraction, Eila P. Järvenpää and Rainer Huopalahti. Supercritical Fluid Extraction of Mycotoxins from Feeds, Rainer Huopalahti and Eila P. Järvenpää. Supercritical Fluid Extraction of Pigments from Seeds of Eschscholtzia californica Cham, Maria L. Colombo and Andrea Mossa. Supercritical Fluid Extraction of Flumetralin from Tobacco Samples, Fernando M. Lanças, Mário S. Galhiane, and Sandra R. Rissato. Supercritical Fluid Extraction and High Performance Liquid Chromatography Determination of Carbendazim in Bee Larvae, José L. Bernal, Juan J. Jiménez, and María T. Martín. Supercritical Fluid Extraction Coupled with Enzyme Immunoassay Analysis of Soil Herbicides, G. Kim Stearman. The Supercritical Fluid Extraction of Drugs of Abuse from Human Hair, Pascal Kintz and Christian Staub. Application of Direct Aqueous Supercritical Fluid Extraction for the Dynamic Recovery of Testosterone Liberated from the Enzymatic Hydrolysis of Testosterone-b-d-Glucuronide, Edward D. Ramsey, Brian Minty, and Anthony T. Rees. Analysis of Anabolic Drugs by Direct Aqueous Supercritical Fluid Extraction Coupled On-Line with High-Performance Liquid Chromatography, Edward D. Ramsey, Brian Minty, and Anthony T. Rees. Detection of Beta-Blockers in Urine and Serum by Solid-PhaseExtraction-Supercritical Fluid Extraction and Gas Chromatography-Mass Spectrometry, Kari Hartonen and Marja-Liisa Riekkola. On-Line SFE-SFC for the Analysis of Fat-Soluble Vitamins and Other Lipids from Water Matrices, Francisco J. Señoráns and Karin E. Markides. Determination of Artemisinin in Artemisia annua L. by Off-Line Supercritical Fluid Extraction and Supercritical Fluid Chromatography Coupled to an Evaporative Light-Scattering Detector, Marcel Kohler, Werner Haerdi, Philippe Christen, and Jean-Luc Veuthey. Analysis of Cannabis by Supercritical Fluid Chromatography with Ultraviolet Detection, Michael D. Cole. Direct Chiral Resolution of Optical Isomers of Diltiazem Hydrochloride by Packed Column Supercritical Fluid Chromatography, Koji Yaku, Keiichi Aoe, Noriyuki Nishimura, Tadashi Sato, and Fujio Morishita. Determination of Salbutamol Sulfate and Its Impurities in Pharmaceuticals by Supercritical Fluid Chromatography, María J. del Nozal, Laura Toribio, José L. Bernal, and Maria L. Serna. Packed Column Supercritical Fluid Chromatographic Determination of Acetaminophen, Propyphenazone, and Caffeine in Pharmaceutical Dosage Forms, Urmila J. Dhorda, Viddesh R. Bari, and M. Sundaresan. Analysis of Shark Liver Oil by Thin-Layer and Supercritical Fluid Chromatography, Christina Borch-Jensen, Magnus Magnussen, and Jørgen Mollerup. Enzymatically Catalyzed Transesterifications in Supercritical Carbon Dioxide, Rolf Marr, Harald Michor, Thomas Gamse, and Helmut Schwab. Transesterification Reactions Catalyzed by Subtilisin Carlsberg Suspended in Supercritical Carbon Dioxide and in Supercritical Ethane, Teresa Corrêa de Sampaio and Susana Barreiros. Enzymatic Synthesis of Peptide in Water-Miscible Organic Solvent/Supercritical Carbon Dioxide, Hidetaka Noritomi. Micronization of a Polysaccharide by a Supercritical Antisolvent Technique, Alberto Bertucco and Paolo Pallado. Rapid Expansion of Supercritical Solutions Technology: Production of Fine Particles of Steroid

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