2-D Proteome Analysis Protocols

With the completion of sequencing projects and the advancement of a— lytical tools for protein identification, proteomics—the study of the expressed part of the genome—has become a major region of the burgeoning field of functional genomics. High—resolution 2—D gels can reveal virtually all p— teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or ext...