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The use of the term "advanced" in the title of this book is somewhat ar­ bitrary and very much relative with respect to time. Many techniques which were considered at the "cutting edge" of ultrastructural methodology just a few years ago are now rou tin ely used in numerous laboratories. One could cite freeze-fracture, cryothin sectioning, or indeed most of the field of scan­ ning electron microscopy as concrete examples. Thus the use of the term "ad­ vanced techniques" must be interpreted ...