G. F. FUEGER Among the many processes in Physiology few appear mo~e inviting to be studied by tracers and external imaging than the variety of the routes of migratory (blood) cells in health and disease. Much emphasis has been placed lately on the methods of labelling of the white blood cells. It is obviously quite important and necessary to refine the methods of leucocytic labelling, particularly to search for ways to label selectively a specific group of white blood cells, but there is also the need to review and keep abreast with the developing knowledge of the white blood cells themselves, especially their behaviour under pathological conditions, as seen by histology and scintigraphy, their biological properties, their immunological characteristics and the mechanisms of the control of leucocytic functions. Similarly, it appears desirable to analyze animal models of inflammation as well as to review the dosimetry and the biodistribution of labelled white blood cells in humans. This book is the result of a cooperative effort to review certain highlights of the physiology of leucocytes, labelled and unlabelled, as a corollary to the effort concerning the labelling of white blood cells. In preparing this book we aim for a better understanding and definition of the goals to be achieved by the successful labelling of the migratory cells of the body. XI CONTRIBUTORS Becker, H. Medizinische Universitaetsklinik der Uni versitaet Graz, Graz, Landeskrankenhaus, haus, Austria Bjurman, B. Department of Radiation Physics, General Hospital, Malmoe, Sweden Chiles, C.
Table of Contents1. Introduction.- 2. Histopathological Infiltrations of Migratory Blood Cells.- 3. Physiology and Pathophysiology of Migratory Blood Cells.- 4. Inflammatory Effectors and Mechanisms of Information Processing for Cellular Reactions and Communication in Regenerative Tissue Morphogenesis by Leucocytes: Chemical Signalling in Poiesis, Recruitment, Taxis, Tropism, and Stasis of Cells.- 5. The Sephadex Model of Acute Inflammation.- 6. Techniques of Cell Handling and Viability Testing.- Editor’s Note: Assays of Migratory Activity.- 7. Radiolabelling Neutrophils Current Accomplishments and Future Possibilities.- Editor’s Note: Chemotactic Agents.- 8. Labelling Blood Cells with 111-In-Tropolonate.- 9. A New Granulocyte Separation and Labelling Technique.- 10. Clinical Applications of 111-In-Labelled Granulocytes.- 11. Phagocytotic Labelling of Migratory Blood Cells and Its Clinical Applications.- 12. Microdosimetry of Labelled Cells.- 13. Conjugate Counting Applied to the Scintigraphic Quantification of Radionuclide Distributions (Scintimetry).- 14. 111-In Labelled White Blood Cells: Biodistribution and Techniques of In-Vivo Quantification.- 15. Radiation Dosimetry of Labelled Leucocytes.- 16. Antigenic Determinants of Migratory Cells.- 17. Round Table Discussion: Comparative Evaluation of Separation and Labelling Methods of Migratory Blood Cells.- Appendix: Large Scale Techniques for Production and Isolation of Cellular Effector Substances of Regenerative Tissue Morphogenesis by Culturing Cells in Serum-Free, Synthetic Fluids: Design, Preparation And Use of a Novel Medium.