Calcium Measurement Methods

by Verkhratsky Alexei (Editor), Ole H. Petersen (Editor)

Overview

Since the introduction of fluorescent calcium indicators and the subsequent development of capacities for real-time monitoring and imaging of calcium movements in the intact cells studied in isolation, in situ and in vivo, the complex and vital calcium signaling system has been illuminated, proving calcium signals to be excellent universal reporters of cellular activity. In "Calcium Measurement Methods", a team of renowned experts in the field contribute methods dedicated to calcium imaging in neural cells with a balance between the main principles of calcium imaging and specific applications of the technique to neural tissues. The book's contents show how the combination of different indicators and recently developed microscopic techniques lead to spectacular successes in the imaging of neural cells in the living brain in both physiological and pathophysiological models. As a volume in the successful Neuromethods™ series, the chapters provide authoritative reviews including up-to-date detailed prools of the most commonly used approaches in the field.

Cutting-edge and concise, "Calcium Measurement Methods" serves as an ideal reference to aid the many scientists worldwide engaged in calcium imaging of the nervous system.

Product Details

ISBN-13:	9781607614753
Publisher:	Springer-Verlag New York, LLC
Publication date:	12/17/2009
Series:	Neuromethods , #43
Edition description:	2010
Pages:	256
Product dimensions:	7.20(w) x 10.10(h) x 0.90(d)

Table of Contents

Preface to the Series v

Preface vii

Contributors xi

1 Principles of the Ca²⁺ Homeostatic/Signalling System Alexei Verkhratsky Ole H. Petersen 1

2 Ca²⁺ Recordings: Hardware and Software (From Microscopes to Cameras) Emil C. Toescu Jeremy Graham 13

3 Ca²⁺ Imaging: Principles of Analysis and Enhancement Fabio Mammano Mario Bortolozzi 57

4 Bioluminescent Ca²⁺ Indicators Laura Fedrizzi Marisa Brini 81

5 Monitoring Calcium Levels With Genetically Encoded Indicators Olga Garaschuk Oliver Griesbeck 101

6 Intracellular Calcium-Sensitive Microelectrodes Roger C. Thomas 119

7 Ca²⁺ Caging and Uncaging Shin Hye Kim Myoung Kyu Park 127

8 Ca²⁺ Imaging of Intracellular Organelles: Endoplasmic Reticulum Robert Blum Ole H. Petersen Alexei Verkbratsky 147

9 Ca²⁺ Imaging of Intracellular Organelles: Mitochondria Luc?a N?ñez Carlos Villalobos Mar?a Teresa Alonso Javier Garc?a-Sancho 169

10 Ca²⁺ Imaging of Dendrites and Spines Knut Holthoff 189

11 In Vivo ²⁺ Imaging of the Living Brain Using Multi-cell Bolus Loading Technique Gerhard Eichhoff Yury Kovalchuk Zsuzsanna Varga Alexei Verkhratsky Olga Garaschuk 205

12 Ca²⁺ Imaging of Glia Christian Lohr Joachim W. Deitmer 221

Index 251