Calcium Techniques: A Laboratory Manual

Life begins with a surge of calcium ions (Ca2+) at fertilization, and thereafter, Ca2+ signaling influences nearly every aspect of mammalian development and physiology, from gene expression and cell proliferation to muscle contraction and nerve impulses. To create spatiotemporally distinct Ca2+ signals, cells use a variety of mechanisms to recognize, transport, and buffer Ca2+. Thus, a diverse range of reliable experimental techniques is necessary to study the movement of Ca2+ and the vario...