Caspases,Paracaspases, and Metacaspases: Methods and Protocols

by Peter V. Bozhkov, Guy Salvesen

Overview

Caspases, Paracaspases, and Metacaspacses: Methods and Prools is a collection of laboratory prools covering current methods that are employed to measure and detect activities of these proteases in diverse biological systems, ranging from unicellular organisms to mammals. Broken into two parts, the first part focuses on methods to measure, detect, and inhibit activation and activity of a subset of or specific caspases in vitro and in several model systems and organisms, primarily in the context of programmed cell death. The second part of the book provides experimental prools for purification and in vitro and in vivo analysis of yeast, protozoan and plant metacaspases, as well as of a human paracaspase MALT1. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Authoritative and practical, Caspases, Paracaspases, and Metacaspacses: Methods and Prools seeks to aid scientists easy-to-follow techniques.

Product Details

ISBN-13:	9781493903566
Publisher:	Springer New York
Publication date:	02/25/2014
Series:	Methods in Molecular Biology , #1133
Edition description:	2014
Pages:	266
Product dimensions:	7.01(w) x 10.00(h) x 0.04(d)

Table of Contents

Part I: Caspases

1. General in vitro Caspase Assay Procedures

Dave Boucher, Catherine Duclos, Jean-Bernard Denault

2. Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Specificity

Marcin Poręba, Aleksandra Stróżyk, Paulina Kasperkiewicz, Marcin Drąg

3. Global Identification of Caspase Substrates using PROTOMAP (PROtein TOpography and Migration Analysis Platform)

Melissa M. Dix, Gabriel M. Simon, Benjamin F. Cravatt

4. Caspase-2 Prools

Loretta Dorstyn and Sharad Kumar

5. Caspase-14 Prools

Mami Yamamoto-Tanaka and Toshihiko Hibino

6. Caspase Prools in Caenorhabditis elegans

Eui Seung Lee & Ding Xue

7. Detecting Caspase Activity in Drosophila Larval Imaginal Discs

Caitlin E. Fogarty and Andreas Bergmann

8. Methods for the Study of Caspase Activation in the Xenopus laevis Oocyte and Egg Extract

Francis McCoy, Rashid Darbandi, and Leta K. Nutt

9. Caspase Prools in Mice

Varsha Kaushal, Christian Herzog, Randy S. Haun, and Gur P. Kaushal

10. Measurement of Caspase Activation in Mammalian Cell Cultures

Magnus Olsson and Boris Zhivotovsky

Part II: Paracaspases and Metacaspases

11. Detection and Measurement of Paracaspase MALT1 Activity

Stephan Hailfinger, Christiane Pelzer, and Margot Thome

12. Leishmania Metacaspase: an Arginine-Specific Peptidase

Ricardo Martin, Iveth Gonzalez and Nicolas Fasel

13. Purification, Characterization, and Crystallisation of Trypanosoma Metacaspases
Karen McLuskey, Catherine X. Moss, and Jeremy C. Mottram

14. Monitoring the Proteostasis Function of the Saccharomyces cerevisiae Metacaspase Yca1

Amit Shrestha, Robin E. C. Lee, and Lynn A. Megeney

15. Plant Metacaspase Activation and Activity

Elena A. Minina, Simon Stael, Frank Van Breusegem, and Peter V. Bozhkov

16. Preparation of Arabidopsis thaliana Seedling Proteomes for Identifying Metacaspase Substrates by N-terminal COFRADIC

Liana Tsiatsiani, Simon Stael, Petra Van Damme, Frank Van Breusegem, and Kris Gevaert