Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods

by David M. Chenoweth (Editor)

Overview

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods, Volume 641 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include caged cyclopropanes with improved tetrazine ligation kinetics, an analysis of metabolically labeled inositol phosphate messengers by NMR, cell-permeant caged inositol pyrophosphates for probing β-cells, imaging phospholipase D activity with clickable alcohols via transphosphatidylation, fluorescent biorthogonal labeling of class B GPCRs in live cells, near-infrared photoactivatable nitric oxide donors with integrated photoacoustic monitoring, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Includes the latest information on retinoid signaling pathways

Product Details

ISBN-13:	9780128211557
Publisher:	Elsevier Science & Technology Books
Publication date:	07/24/2020
Series:	Methods in Enzymology , #641
Sold by:	Barnes & Noble
Format:	eBook
Pages:	526
File size:	53 MB
Note:	This product may take a few minutes to download.

About the Author

David Chenoweth is at University of Pennsylvania, PA, USA

Table of Contents

- Enzyme- or light-triggered cyclopropenes for bioorthogonal ligation Ting Jiang and Scott T. Laughlin - Analysis of metabolically labeled inositol phosphate messengers by NMR Robert Puschmann, Robert K. Harmel and Dorothea Fiedler - Photo-releasable derivatives of inositol pyrophosphates Sebastian Hauke, Tamara Bittner, Henning J. Jessen and Carsten Schultz - IMPACT: Imaging phospholipase D activity with clickable alcohols via transphosphatidylation Timothy W. Bumpus, Dongjun Liang and Jeremy M. Baskin - Fluorescent bioorthogonal labeling of class B GPCRs in live cells Srikanth Kumar Gangam and Qing Lin - Near-infrared photoactivatable nitric oxide donors with photoacoustic readout Effie Y. Zhou, Hailey J. Knox, Christopher J. Reinhardt, Gina Partipilo and Jefferson Chan - H2S donors with optical responses Michael D. Pluth, Yu Zhao and Matthew M. Cerda - Visualization of oxidative stress-induced carbonylation in live mammalian cells Kamalika Mukherjee, Tak Ian Chio and Susan L. Bane - Receptor-based fluorescent sensors constructed from ribonucleopeptide Skun Nakano, Hiroaki Konishi and Takashi Morii - Conformationally restrained pentamethine cyanines and use in reductive single molecule localization microscopy Siddharth S. Matikonda, Ralph Götz, Ryan McLaughlin, Markus Sauer and Martin J. Schnermann - A near-infrared light-mediated cleavable linker strategy using the heptamethine cyanine chromophore Michael P. Luciano, Saghar Nourian, Alexander P. Gorka, Roger R. Nani, Tadanobu Nagaya, Hisataka Kobayashi and Martin J. Schnermann - Quantitative measurement of cytosolic penetration using the chloroalkane penetration assay Kirsten Deprey and Joshua A. Kritzer - Cell-penetrating and mitochondrion-targeting molecules George Appiah Kubi and Dehua Pei - A photocaged DNA nanocapsule for delivery and manipulation in cells Yihong Feng, Takeshi Tohgasaki, Yasuyuki Shitomi, Hiroshi Sugiyama and Masayuki Endo - A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells Esther Braselmann and Amy E. Palmer - Enzymatic covalent labeling of RNA with RNA transglycosylation at guanosine (RNA-TAG) Kayla N. Busby and Neal K. Devaraj - Assaying RNA solvent accessibility in living cells with LASER Chao Feng and Robert C. Spitale - Split T7 RNA polymerase biosensors to study multiprotein interaction dynamics Jeffrey A. Dewey and Bryan C. Dickinson - Cross-linking cellular nucleic acids via a target-directing double click reagent Masayuki Tera and Nathan W. Luedtke - Click chemistry-based amplification and detection of endogenous RNA and DNA molecules in situ using clampFISH probes Sepideh Tavakoli, Yifang Liu, Jacob Potts and Sara H. Rouhanifard - Quantification of protein delivery in live cells using fluorescence correlation spectroscopy Susan L. Knox, Angela Steinauer, Garrett Alpha-Cobb, Adam Trexler, Elizabeth Rhoades and Alanna Schepartz

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From the Publisher

This new volume in the Methods in Enzymology series focuses on chemical tools for imaging, manipulating and tracking biological systems