Cilia: Methods and Protocols

by Peter Satir, Sïren Tvorup Christensen

Overview

This volume is the result of an explosion of molecular-based research on Cilia, which began with the discovery of the universality of intraflagellar transport (IFT) and ciliary genomics/proteomics. The chapters in this book cover topics such as: high resolution imaging and functional characterization of sensory and primary cilia in mammalian cells and zebrafish, methods to study ciliary-mediated chemoresponse in Paramecium, and methods to study centrosomes and cilia in C. elegans and Drosophila. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Cilia: Methods and Prools is broad and covers motile, sensory, and primary cells. It is a valuable resource to anyone interested in entering the field of ciliary biology using model organisms, including flagellate algae, ciliates, planaria, nematodes, insects, zebrafish, and mammalian cells.

Product Details

ISBN-13:	9781493981397
Publisher:	Springer New York
Publication date:	06/09/2018
Series:	Methods in Molecular Biology , #1454
Edition description:	Softcover reprint of the original 1st ed. 2016
Pages:	259
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Methods for Studying Ciliary Import Mechanisms.- Targeting of ASH Domain-Containing Proteins to the Centrosome.- Morphological and Functional Characterization of the Ciliary Pocket by Electron and Fluorescence Microscopy.- Methods to Study Interactions between Ciliogenesis and Autophagy.- Recombinant Reconstitution and Purification of the IFT B Core Complex from Chlamydomonas reinhardtii.- Methods for Studying Movement of Molecules within Cilia.- A FRAP-Based Method for Monitoring Molecular Transport in Ciliary Photoreceptor Cells In Vivo.- Kymographic Analysis of Transport in an Individual Neuronal Sensory Cilium in Caenorhabditis elegans.- Visualization and Manipulation of Cilia and Intraciliary Calcium in the Zebrafish Left-Right Organizer.- Methods for Studying Ciliary-Mediated Chemoresponse in Paramecium.- STED and STORM Superresolution Imaging of Primary Cilia.- CLEM Methods forStudying Primary Cilia.- Methods for Visualization of Neuronal Cilia.- Methods to Study Centrosomes and Cilia in Drosophila.- Analysis of Axonemal Assembly during Ciliary Regeneration in Chlamydomonas.- Planaria as a Model System for the Analysis of Ciliary Assembly and Motility.