CRISPR Gene Editing: Methods and Protocols

This detailed volume guides readers through strategic planning and user—friendly guidelines in order to select the most suitable CRISPR—Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods i...