Enzymes of Molecular Biology / Edition 1

by Michael M. Burrell

Overview

The scientist' s understanding of the cell at the molecular level has advanced rapidly over the last twenty years. This improved understa- ing has led to the development of many new laboratory methods that increasingly allow old problems to be tackled in new ways. Thus the modern scientist cannot specialize in just one field of knowledge, but must be aware of many disciplines. To aid the process of investigation, the Methods Molecular Biology series has brought together many prools and has highlighted the useful variations and the pitfalls of the different methods. However, prools frequently cannot be simply taken from the shelf. Thus the starting sample for a chosen prool may be unavailable in the correct state or form, or the products of the procedure require a different sort of processing. Therefore the scientist needs more detailed information on the nature and requirements of the enzymes being used. This information, though usually available in the literature, is often widely dispersed and frequently occurs in older volumes of journals; not everyone has comprehensive library facilities available. Also many scientists searching out such information are not trained enzymologists and may be unaware of some of the parameters that are important in a specific enzyme reaction.

Product Details

ISBN-13:	9780896032347
Publisher:	Springer-Verlag New York, LLC
Publication date:	05/22/1993
Series:	Methods in Molecular Biology , #16
Edition description:	1993
Pages:	370
Product dimensions:	6.10(w) x 9.25(h) x 0.03(d)

Table of Contents

Nucleases: An Overview. Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1). DNA Polymerases (EC 2.7.7.7). Taq Polymerases (EC 2.7.7.7): With Particular Emphasis on Its Use in PCR Prools. Eukaryotic Nuclear RNA Polymerases (EC 2.7.7.6). Reverse Transcriptase (EC 2.7.7.49): The Use of Cloned Moloney Murine Leukemia Virus Reverse Transcriptase to Synthesize DNA from RNA. Terminal Deoxyribonucleotidyl Transferase (EC 2.7.7.31). Restriction Enzymes. DNA Methyltransferases (EC 2.1.1.72 and EC 2.1.1.73). DNA and RNA Ligases (EC 6.5.1.1, EC 6.5.1.2, and EC 6.5.1.3). The BAL 31 Nucleases (EC 3.1.11). Mung-Bean Nuclease 1 (EC 3.1.30.1). RNase A (EC 3.1.27.5). Pronase (EC 3.4.24.4). Proteolytic Enzymes for Peptide Production. Proteinase K (EC 3.4.21.14). Carboxypeptidase Y (EC 3.4.16.1). Aminopeptidases: Aminopeptidase M (EC 3.4.11.2), Pyroglutamate Aminopeptidase (EC 3.4.19.3), and Prolidase (EC 3.4.13.9). Alkaline Phosphatase (EC 3.1.3.1). Polynucleotide Kinase (EC 2.7.1.78). Index.

Editorial Reviews

Amasses key information on a broad range of enzymes commonly used as tools in molecular biology, condensing scattered sources for ease of reference, and making data accessible to nonenzymologists designing an experiment. After an overview of nucleases, 19 chapters give background on selected enzymes (nucleic acid- and protein-modifiers), discuss essential parameters for achieving optimized reactions, and provide exemplary practical procedures and protocols. Plastic comb binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Booknews

An essential resource for every biochemist and scientist in molecular biology. -Unlisted Drugs

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