Eosinophils: Methods and Protocols

by Garry M. Walsh (Editor)

Overview

In Eosinophil: Methods and Protocols, experts in the field of eosinophil biology comprehensively provide detailed methodological insight into the study of this fascinating cell. This book is aimed at a diverse range of basic and clinical scientists who wish to work with eosinophils or who require an update of their knowledge or to gain the information required to study a function of the eosinophil different to their current area of enquiry.​ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Eosinophil: Methods and Protocols seeks to aid scientist in the discovery of new hypotheses and for further examination of this intriguing cell.

Product Details

ISBN-13:	9781071610978
Publisher:	Springer US
Publication date:	01/24/2022
Series:	Methods in Molecular Biology , #2241
Edition description:	Second Edition 2021
Pages:	297
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Eosinophil Overview - Structure, Biological Properties And Key Functions.- Eosinophil Purification From Peripheral Blood.- Eosinophil Purification From Human Bone Marrow.- CD34(+) Eosinophil-Lineage-Committed Cells In The Mouse Lung.- Eosinophil Cell Lines.- Cell Signalling In Eosinophil During Human Eosinophil Differentiation.- Eosinophil Intracellular Signalling – Apoptosis.- Identification Of Human Eosinophils In Whole Blood By Flow Cytometry.- Isolation And Functional Assessment Of Eosinophil Crystalloid Granules.- Eosinophil Chemotaxis.- Human Eosinophil Shape Change And Secretion.- Human Eosinophil Adhesion And Receptor Expression.- Adhesion Of Eosinophils To Endothelial Cells Or Substrates Under Flow Conditions.- Human Eosinophil Transmigration.- Measurement Of Eosinophil Kinetics In Healthy Volunteers.- Assays Of Eosinophil Apoptosis And Phagocytic Uptake.- Clinical Measurement Of Eosinophil Numbers In Eosinophilic Conjunctivitis.- Qualitative And Quantitative Studies Of Eosinophils In Parasitic Infections.- Interactions Of Eosinophils With Nerves.- Eosinophils Interaction With Mast Cells: The Allergic Effector Unit.- Eosinophil Interactions: Antigen Presentation.- Eosinophils And Respiratory Virus Infection: A Dual-Standard Curve Qrt-PCR-Based Method For Determining Virus Recovery From Mouse Lung Tissue.- Antimicrobial Activity Of Human Eosinophil Granule Proteins.- Eosinophils And The Ovalbumin Mouse Model Of Asthma.- Mutant Mice And Animal Models Of Allergic Disease.- Murine Models Of Eosinophilic Leukemia - A Model Of FIP1L1-PDGFRa Initiated Chronic Eosinophilic Leukemia/Systemic Masytosis.