Flow Cytometry Protocols

This fifth edition volume expands on the previous editions by presenting readers with the latest developments and emerging methodologies in cytometry. The chapters in this book cover cytometry basics such as lasers for cytometry, metrics that can be used to evaluate spillover spreading, and the process of panel design and iterative optimization for spectral flow cytometry; novel methodologies such as image-enabled cell sorting, co-staining of fluorochrome-conjugated and oligonucleotide-conj...