How to Use a Spectrophotometer

How to Use a Spectrophotometer

by Richard A. Neuhaus

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The Neuhaus Science Projects Guide, How to Use a Spectrophotometer, is written for the average high school student in grades 9 through 12.

This guide booklet is written to help students succeed with science projects that require the use of a spectrophotometer. It provides details on the use of the equipment without going into the technical details behind the operations. Preparation of solutions, setups, and procedures necessary to obtain the data needed are detailed. Colored images, graphs, and other illustrations augment the text.

Topics discussed:

How to Use a Spectrophotometer:--

How Much Is in It? • Light and Color • The Choice of Instrument • Old and New Models • The Guts of a Spectrophotometer • How the System Works – Guts and All • Transmittance • The Analytical Wavelength • How to Find the Analytical Wavelength of a Substance • Generic Operating Instructions • The Importance of the Blank • Plastic Versus Glass • Matching Cuvettes • Sample Concentration.

Quantitative Analysis:--

Understanding the Standard Curve • Preparation of Solutions • The Setup • Procedure • Graphing the Standard Curve • How to Use the Standard Curve.

Protein Analysis:--

Preparation of Solutions • The Setup • Procedure • Testing an Unknown Protein Sample • Cleaning Up • Other Methods of Protein Assay.

The Filter Colorimeter


Product Details

BN ID: 2940157823689
Publisher: Gormley Publishing
Publication date: 01/14/2016
Sold by: Barnes & Noble
Format: NOOK Book
Pages: 50
File size: 8 MB

About the Author

Richard Newhouse holds a Master of Science degree specializing in cellular and molecular biology from Cleveland State University, where he has done research in the fields of endocrinology and the aging process. In addition, he has an undergraduate teaching degree in biology from Waynesburg University, and a major in chemistry. He also holds a Master of Education degree from Rutgers University.

Mr. Newhouse has had 32 years of broad teaching experience in secondary education at all grade levels, 7-12, in the subjects of general science, biology, and chemistry. He has written numerous books on science activities – some published under his ancestral name "Neuhaus." He was a volunteer judge at regional and state level science fairs in Ohio. Many of his students won science project awards and later chose science careers.

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