Light Microscopy: Methods and Protocols

This volume addresses up—to—date light microscopy approaches and toolsets offered for live— or fixed—cell observations. The imaging strategies discussed in this book include confocal laser scanning and spinning disk confocal microscopy, FRET, FRAP, and laser microsurgery experiments. Chapters also describe the use of these imaging methodologies to study properties of a multitude of biomolecular targets in a broad range of model systems ranging from bacteria over tissue to whole animal imagi...