Methods in Kidney Cell Biology Part B

by Thomas Weimbs

Overview

Methods in Kidney Cell Biology, Part B, Volume 154 represents state-of-the-art techniques in renal research that are ideal for veterans, graduate students, postdoctoral fellows, clinical scientists and principal investigators. Topics in the new release include Single glomerular proteomics – a novel method in translational glomerular cell biology, Measurement of cytosolic and intraciliary calcium in live cells, Differentiation of human kidney organoids from pluripotent stem cells, Quantifying autophagic flux in kidney tissue using structured illumination microscopy, the Generation of primary cells from ADPKD and normal human kidneys, ADPKD cell proliferation and Cl-dependent fluid secretion, In vitro cyst formation of ADPKD cells, and much more.

Product Details

ISBN-13:	9780128203354
Publisher:	Elsevier Science
Publication date:	09/01/2019
Series:	Methods in Cell Biology , #154
Pages:	254
Product dimensions:	7.50(w) x 9.25(h) x (d)

About the Author

Dr. Weimbs received his doctoral degree from the Department of Biochemistry of the University of Cologne, Germany, in 1993. He conducted postdoctoral research with Keith Mostov at the Department of Anatomy, University of California at San Francisco until 1999 where he investigated the role of SNARE proteins in membrane trafficking and epithelial cell polarity. In 1999, he joined the Department of Cell Biology in the Lerner Research Institute of the Cleveland Clinic as an Assistant Professor where he established his own research laboratory. Besides continuing his work on SNAREs and epithelial cell polarity his laboratory began to investigate molecular mechanisms underlying polycystic kidney disease (PKD). In 2005, Dr. Weimbs moved his lab to the University of California in Santa Barbara where he is currently a Professor in the Department of Molecular, Cellular, and Developmental Biology.
Research on PKD in Dr. Weimbs’ laboratory has contributed to our understanding of the molecular pathogenesis and the function of polycystin-1, the protein affected in this disease. These contributions include the roles of mTOR and STAT signaling in PKD. Recent work has focused on developing new kidney-targeted therapeutics, the role of metabolic changes and tubular crystal deposition in PKD disease progression, and the use of dietary interventions for PKD therapy.

Table of Contents

1. Single glomerular proteomics – a novel tool for translational glomerular cell biology Markus Rinschen  2. Kidney harvesting and metabolite extraction for metabolomics studies in rodents Maria Irazabal Mira  3. Optical tissue clearing and immunolabeling in kidney research David Ellison 4. Urinary extracellular vesicles as a source of biomarkers reflecting renal cellular biology in human disease Roman Müller  5. Intravital Visualization of the Primary Cilium, Tubule Flow, and Innate Immune Cells in the Kidney Utilizing an Abdominal Window Imaging Approach Bradley Yoder 6. Novel fluorescence techniques to quantitate renal cell biology Janos Peti-Peterdi  7. Manipulation of Renal Gene Expression Using Oligonucleotides Vishal Patel 8. Methods for renal lineage tracing: in vivo and beyond Thomas Carroll 9. In Vivo Magnetic Resonance Imaging Techniques for Structural and Functional Characterization of Murine Model Kidneys Tim Kline 10. In vivo analysis of renal epithelial cells in zebrafish Zhaoxia Sun 11. Visualizing gene expression during zebrafish pronephros development and regeneration Rebecca Wingert 12. Drosophila melanogaster and its nephrocytes – a versatile model for glomerular research Paul Brinkkotter

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From the Publisher

Presents an up-to-date collection of methods for the investigation of renal cell biology as discussed by experts in the field