Methods in Stem Cell Biology - Part B

by Ilio Vitale (Editor), Gwenola Manic (Editor), Lorenzo Galluzzi (Editor)

Overview

Methods in Cancer Stem Cell Biology: Part B, Volume 171 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more.

Product Details

ISBN-13:	9780323900188
Publisher:	Elsevier Science
Publication date:	08/09/2022
Series:	Methods in Cell Biology , #171
Pages:	260
Product dimensions:	7.50(w) x 9.25(h) x (d)

About the Author

Dr Ilio Vitale is an esteemed oncologist. He is a member of the University of Rome and associated to the Regina Elena National Cancer Institute based in Italy.

Gwenola Manic received her Ph.D. from the University of Paris Sud/Paris XI (France) in 2012 studying the impact of the design of HIV-1-derived vectors and DNA repair machinery on lentiviral expression. At present she is working as a postdoctoral fellow at the Department of Biology at the Università degli Studi di Roma “Tor Vergata” in Rome (Italy) on the contribution of chromosomal instability in tumorigenesis and the therapeutic targeting of cancer stem cells.

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.

Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Table of Contents

1. Multiple Gene Knockdown Strategies for Investigating the Properties of Human Leukemia Stem Cells and Exploring New Therapies
Xiaoyan Jiang
2. Assessment of CD133, EpCAM, and CD44 in human and murine cancer cell by real-time qPCR
Guillermo Mazzolini
3. Reverse Phase Protein Array in cancer stem cells
Michele Signore
4. Isolation of cancer stem cells from squamous cell carcinoma
Sanjeev K. Centre (TMC), Advanced Centre for Treatment and Research and Education in Cancer (ACTREC),
5. Immunoblotting-assisted assessment of JAK2 signaling in leukemic stem cells
Niccolò Bartalucci
6. Isolation of cancer stem cells from based on aldehyde dehydrogenase activity
Paola Marcato
7. Generation of neuronal/glial mixed cultures from human induced pluripotent stem cells (hiPSCs)
Angela Gritti
8. Immunohistochemistry-based analysis of EMT markers in mammary tumors
Diwakar Pattabiraman
9. Immunofluorescence staining of colorectal cancer patient-derived organoids
Joseph L. Regan
10. Isolation of murine bone marrow hematopoietic stem and progenitor cell populations via flow cytometry
Michael A. Rieger
11. In vitro and in vivo limiting dilution assay of hepatocellular carcinoma cells
Bin Wang Sr., Yingying Lai and Xi Zheng
12. Methods to isolate adipose tissue-derived stem cells
Barbara Zavan, Paolo Pinton, Leticia Ferroni, Chiara Gardin and Francesco De Francesco
13. In vivo assessment of the tumorigenic and metastatic potential of the cancer stem cell fraction in breast cancer cells
Antonella Sistigu

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From the Publisher

Edited by one of the best scientists in the field, this series presents the latest updates in the field of cell biology