ISBN-10:
9048197341
ISBN-13:
9789048197347
Pub. Date:
10/28/2010
Publisher:
Springer Netherlands
Microbial Plant Pathogens-Detection and Disease Diagnosis:: Fungal Pathogens, Vol.1 / Edition 1

Microbial Plant Pathogens-Detection and Disease Diagnosis:: Fungal Pathogens, Vol.1 / Edition 1

by P. Narayanasamy

Hardcover

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Overview

Morphological, biological, biochemical and physiological characteristics have been used for the detection, identification and differentiation of fungal pathogens up to species level. Tests based on biological characteristics are less consistent. Immunoassays have been shown to be effective in detecting fungal pathogens present in plants and environmental samples. Development of monoclonal antibody technology has greatly enhanced the sensitivity and specificity of detection, identification and differentiation of fungal species and varieties/strains. Nucleic acid-based techniques involving hybridization with or amplification of unique DNA have provided results rapidly and reliably. Presentation of a large number of protocols is a unique feature of this volume.

Product Details

ISBN-13: 9789048197347
Publisher: Springer Netherlands
Publication date: 10/28/2010
Edition description: 2011
Pages: 291
Product dimensions: 6.10(w) x 9.25(h) x 0.24(d)

Table of Contents

Volume 1
Chapter 1 Introduction
1.1 Microbial plant pathogens as a major limiting factor of crop production
1.2 Discovery of fungi as plant pathogens
1.3 Detection of fungal plant pathogens and disease diagnosis
References
Chapter 2 Detection of Fungal Pathogens in Plants
2.1 Detection of fungal pathogens in plant organs
2.1.1 Biological methods
2.1.2 Pathogenicity and host range
2.1.3 Biochemical methods
2.1.4 Immunoassays
2.1.5 Nucleic acid-based detection techniques
2.2 Detection of fungal pathogens in seeds and planting materials
2.2.1 Detection of fungal pathogens in seeds
2.2.2 Detection of fungal pathogens in propagative planting materials
2.2.3 Detection of fungal pathogens in postharvest produce
Appendix
References
Chapter 3 Detection of Fungal Pathogens in the Environment
3.1 Detection of fungal pathogens in soil
3.1.1 Bioassays
3.1.2 Immunoassays
3.1.3 Nucleic acid-based techniques
3.2 Detection of fungal pathogens in water
3.2.1 Immunoassays
3.2.2 Nucleic acid-based techniques
3.3 Detection of fungal pathogens in air
3.4 Detection of fungal pathogens in alternative host plants
Appendix
References
Chapter 4 Assessment of Variability in Fungal Plant Pathogens
4.1 Methods of assessment of variability in fungal pathogens
4.1.1 Assessment of variations in biological characteristics
4.1.2 Assessment of variations in biochemical characteristics
4.1.3 Assessment of variations in immunological characteristics
4.1.4 Assessment of variations in genomic characteristics
4.2 Assessment of variability in sensitivity to chemicals
4.2.1 Assessment of variations in biological characteristics
4.2.2 Assessment of variations in genetic characteristics
Appendix
References
Chapter 5 Diagnosis of Fungal Diseases of Plants
5.1 Choice of diagnostic tests for fungal pathogens
5.1.1 Conventional methods
5.1.2 Molecular methods
5.2 Agencies involved in disease diagnosis
5.2.1 Disease diagnostic centers
5.2.2 Plant quarantines
References

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