Neural Development: Methods and Protocols

Neural Development: Methods and Protocols

Paperback(Softcover reprint of the original 1st ed. 2013)

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Product Details

ISBN-13: 9781493960187
Publisher: Springer-Verlag New York, LLC
Publication date: 08/23/2016
Series: Methods in Molecular Biology , #1018
Edition description: Softcover reprint of the original 1st ed. 2013
Pages: 347
Product dimensions: 7.01(w) x 10.00(h) x 0.03(d)

Table of Contents

Part I: Neuron and Glial Cell Culture

1. Isolation and Maintenance of Cortical Neural Progenitor Cells In Vitro

Mi-Yeon Kim, Byoung-San Moon, and Kang-Yell Choi

2. Neural Induction with a Dopaminergic Phenotype from Human Pluripotent Stem Cells Through a Feeder-Free Floating Aggregation Culture

Asuka Morizane, Daisuke Doi, and Jun Takahashi

3. Nucleoside Analog Labeling of Neural Stem Cells and Their Progeny

Erik DeBoer and Mladen-Roko Rasin

4. Culture of Dissociated Hippocampal Neurons

Yun Peng, Wen-Cheng Xiong, and Lin Mei

5. Culturing Mouse Cerebellar Granule Neurons

Tharakeswari Selvakumar and Daniel L. Kilpatrick

6. Preparation of Primary Cultured Dopaminergic Neurons from Mouse Brain

Won-Seok Choi, Hyung-Wook Kim, and Zhengui Xia

7. Culturing Astoryctes from Postnatal Rats

Yangyang Huang and Cheryl Dreyfus

8. Culturing Oligodendrocyte Lineage Cells from Neonatal Rats

Clifton G. Fulmer and Cheryl F. Dreyfus

9. Isolation and Culture of Schwann Cells

Yanmei Tao

Part II: Gene Expression and Analysis

10. DNA Transfection: Calcium Phosphate Method

Munjin Kwon and Bonnie L. Firestein

11. The Gene-Gun Approach for Transfection and Labeling of Cells in Brain Slices

Anna Dunaevsky

12. Lentiviral Vector Production, Titration, and Transduction of Primary Neurons

Baojin Ding and Daniel L. Kilpatrick

13. In-Ovo Electroporation in Embryonic Chick Spinal Cords

Hui Wang and Michael P. Matise

14. Gene Transfer in Developing Chick Embryos: In Ovo Electroporation

Emma K. Farley

15. In Utero Electroporation in Mice

Chunlei Wang and Lin Mei

16. General Introduction to In Situ Hybridization Protocol Using Non-Radioactively Labeled Probes to Detect mRNAs on Tissue Sections

Daehoon Lee, Shan Xiong, and Wen-Cheng Xiong

17. Immunofluorescence Staining with Frozen Mouse or Chick Embryonic Tissue Sections

Hui Wang and Michael P. Matise

18. β-Galactosidase Staining of LacZ Fusion Proteins in Whole Tissue Preparations

Margaret A. Cooper and Renping Zhou

19. Chromatin Immunoprecipitation Assay of Brain Tissues Using Percoll Gradient-Purified Nuclei

Baojin Ding and Daniel L. Kilpatrick

20. In Vivo Dual Luciferase Reporter Assay with Chick Neural Tube In Ovo Electroporation System

Hui Wang and Michael P. Matise

Part III: Axon Pathways and Synapses

21. Growth Cone Collapse Assay

Xin Yue, Alexander I. Son, and Renping Zhou

22. The Stripe Assay: Studying Growth Preference and Axon Guidance on Binary Choice Substrates In Vitro

Markus Weschenfelder, Franco Weth, Bernd Knöll, and Martin Bastmeyer

23. Microcontact Printing of Substrate-Bound Protein Patterns for Cell- and Tissue Culture

Martin Fritz and Martin Bastmeyer

24. Semi-Automated Analysis of Dendrite Morphology in Cell Culture

Eric S. Sweet, Chris L. Langhammer, Melinda K. Kutzing, and Bonnie L. Firestein

25. Monitoring Synaptic Plasticity by Imaging AMPA Receptor Content and Dynamics on Dendritic Spines

Hiroshi Makino and Bo Li

26. Morphological Analysis of Neuromuscular Junctions by Immunofluorescent Staining of Whole-Mount Mouse Diaphragms

Haitao Wu and Lin Mei

Part IV: Morphological Analyses of the Developing Nervous System

27. Routine Histology Techniques for the Developing and Adult Central Nervous System

W. Geoffrey McAuliffe

28. Cryosectioning

Alexander I. Son, Katie Sokolowski, and Renping Zhou

29. The Golgi-Cox Method

Gitanjali Das, Kenneth Reuhl, and Renping Zhou

30. Neuroanatomical Tract-Tracing Using Biotinylated Dextran Amine

Nikolai E. Lazarov

31. Retrograde Tracing Technique for Neonatal Animals

Kengo Funakoshi, Akira Yoshikawa, and Yoshitoshi Atobe

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