This book presents the fundamental physics of optical interferometry as applied to biophysical, biological and medical research. Interference is at the core of many types of optical detection and is a powerful probe of cellular and tissue structure in interfererence microscopy and in optical coherence tomography. It is also the root cause of speckle and other imaging artefacts that limit range and resolution. For biosensor applications, the inherent sensitivity of interferometry enables ultrasensitive detection of molecules in biological samples for medical diagnostics. In this book, emphasis is placed on the physics of light scattering, beginning with the molecular origins of refraction as light propagates through matter, andthen treating the stochastic nature of random fields that ultimately dominate optical imaging in cells and tissue. The physics of partial coherence plays a central role in the text, with a focus on coherence detection techniques that allow information to be selectively detected out of incoherent and heterogeneous backgrounds.
Optical Interferometry for Biology and Medicine is divided into four sections. The first covers fundamental principles, and the next three move up successive scales, beginning with molecular interferometry (biosensors), moving to cellular interferometry (microscopy), and ending with tissue interferometry (biomedical). An outstanding feature of the book is the clear presentation of the physics, with easy derivations of the appropriate equations, while emphasizing "rules of thumb" that can be applied by experimental researchers to give semi-quantitative predictions.
About the Author
David D. Nolte is a professor of Physics at Purdue University, working in the field of holographic optics and biophotonics. He has been elected a Fellow of the Optical Society of America and a Fellow of the American Physical Society. He was a Research Fellow of the Alfred P. Sloan Foundation, and a Presidential Young Investigator of the National Science Foundation. In 2005 he received the Herbert Newby McCoy Award, the highest scientific honor awarded by Purdue University.
David received his baccalaureate from Cornell University in 1981 and his Ph.D from the University of California at Berkeley in 1988. He is the author of over two hundred journal papers, book chapters and encyclopedia articles, and has secured a dozen US patents in applied optics and biophotonics.
David is also the author of the trade non-fiction book Mind at Light Speed published in 2001 by Free Press, which describes the history and technology of optical telecommunications. He is a popular lecturer, receiving the Best Teaching Award for undergraduate teaching in physics, and has been interviewed on public radio and by science magazines on the topics of his scientific research.
Table of Contents
Part I. Fundamentals of Biological Optics
CHAPTER 1. INTERFEROMETRY
CHAPTER 2. DIFFRACTION AND LIGHT SCATTERING
CHAPTER 3. SPECKLE AND SPATIAL COHERENCE
CHAPTER 4. SURFACE OPTICS
Part II. Molecular Interferometry and Biosensors
CHAPTER 5. INTERFEROMETRIC THIN FILM OPTICAL BIOSENSORS
CHAPTER 6. DIFFRACTIONBASED INTERFEROMETRIC BIOSENSORS
CHAPTER 7. INTERFEROMETRIC WAVEGUIDE SENSORS
Part III. Cellular Interferometry
CHAPTER 8. CELL STRUCTURE AND DYNAMICS
CHAPTER 9. INTERFERENCE MICROSCOPY
Part IV. Interferometry of Biological Tissues
CHAPTER 10. LIGHT PROPAGATION IN TISSUE
CHAPTER 11. OPTICAL COHERENCE TOMOGRAPHY
CHAPTER 12. HOLOGRAPHY OF TISSUES
CHAPTER 13. APPENDIX: MATHEMATICAL FORMULAS