Protocols for Macroalgae Research

Protocols for Macroalgae Research


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This book presents a wide range of tested and proven protocols relevant to a number of fields within biotechnology used in laboratory experiments in everyday phycological (seaweed) research.

Product Details

ISBN-13: 9781498796422
Publisher: Taylor & Francis
Publication date: 04/27/2018
Pages: 518
Product dimensions: 6.12(w) x 9.25(h) x (d)

About the Author

Dr. Bénédicte Charrier is a senior scientist from CNRS working at Station Biologique Roscoff, France. She is a specialist of macroalgae morphogenesis and development, with an expertise on the filmentous alga Ectocarpus siliculosus. She is currently the Chair of the European network "Phycomorph", supported by the Cooperation in Science & Technology (COST) Association (2015-2019), which aims to coordinate research in growth, reproduction, and morphogenesis of macroalgae in Europe and in association with Asian and North American laboratories.

Dr. Thomas Wichard is a research group leader at the Institute for Inorganic and Analytical Chemistry of the Friedrich Schiller University Jena. The main focus of his research group is to elucidate the mutualistic interactions between bacteria and the marine macroalga Ulva ("cross-kingdom-cross-talk"). The group applies various methodologies in analytical chemistry, chemical ecology and molecular biology to understand the basis of eco-physiological processes.

Dr. CRK Reddy is a Chief Scientist in Seaweed Biology and Biotechnology Cultivation at CSIR-Central Salt and Marine Chemicals Research Institute in Bhavnagar, India. He is an expert in marine macroalgae, making significant contributions to macroalgae tissue culture and protoplast techniques including genetic improvement of seaweeds, seaweed biomass for chemicals and biofuels, seaweed biodiversity, and seaweed biology and cultivation.

Table of Contents


Section I: Cultivating and preserving seaweeds

Chapter 1 Seaweed in high-energy environments: Protocol

to move Saccharina cultivation offshore

Bela H. Buck and Britta Grote

Chapter 2 Cultivation protocol for Saccharina latissima

Silje Forbord, Kristine Braaten Steinhovden,

Kaia Kjølbo Rød, Aleksander Handå, and Jorunn Skjermo

Chapter 3 Derivation of clonal stock cultures and hybridization

of kelps: A tool for strain preservation and breeding


Inka Bartsch

Chapter 4 Cryopreservation of macroalgae

John G. Day

Chapter 5 Unraveling seaweeds bacteriomes: From field site

to computer screen

Tânia Aires, Gerard Muyzer, Ester A. Serrão, and

Aschwin H. Engelen

Chapter 6 Heavy metal ecotoxicity on the early life history

stages of macroalgae

Pablo P. Leal and Michael Y. Roleda

Chapter 7 A simple protocol for a rapid and consistent

production of a large number of viable protoplasts

from the Ulvophycean species

Vishal Gupta and C.R.K. Reddy

Chapter 8 Purification of sporulation and swarming inhibitors

from Ulva: Application in algal life-cycle controlling

Ralf W. Kessler, Taghreed Alsufyani, and Thomas Wichard

Chapter 9 Preparation of axenic cultures in Ulva (Chlorophyta)

Gianmaria Califano and Thomas Wichard

Section II: Chemical composition

Chapter 10 Biochar production from seaweeds

Loretto Contreras-Porcia, Matías Araya,

Elizabeth Garrido-Ramírez, Cristian Bulboa,

Jean Pierre Remonsellez, Javier Zapata, Camila Espinoza,

and Jorge Rivas

Chapter 11 Identification and quantification of laminarins

in brown algae

Angelika Graiff, Wolfgang Ruth, and Ulf Karsten

Chapter 12 Determination of carbohydrate composition

of macroalgae

Wouter J.J. Huijgen, E.M. Cobussen-Pool, B.F. van Egmond,

and J.W. van Hal

Chapter 13 Quantification of proteins in seaweeds

Carl Safi, Jelle van Leeuwen, Yvette Telleman,

Nicole Engelen-Smit, Lambertus van den Broek,

and Paulien Harmsen

Chapter 14 Comprehensive phytohormone quantification

in the red alga Pyropia yezoensis by liquid

chromatography–mass spectrometry

Takakazu Matsuura, Izumi C. Mori, Yoko Ikeda,

Takashi Hirayama, and Koji Mikami

Chapter 15 Total phenolic content and antioxidant capacity

analysis of seaweed biomass

Xiaoru Hou, Randi Neerup, and Anne-Belinda Bjerre

Chapter 16 Extraction of phycocyanin and phycoerythrin pigments

Stewart William Beattie, Michèle Morançais, Paul Déléris,

Joël Fleurence, and Justine Dumay

Chapter 17 Quantification and localization of reactive oxygen

species in marine macrophytes

Manoj Kumar, Loretto Contreras-Porcia, Nirali M. Kumar,

and Peter J. Ralph

Chapter 18 Metabolomics of intra- and extracellular metabolites

from micro- and macroalgae using GC–MS

and LC–MS

Constanze Kuhlisch, Gianmaria Califano, Thomas Wichard,

and Georg Pohnert

Chapter 19 Preparative extraction of exometabolites

from seaweed surfaces

Florian Weinberger

Chapter 20 Disruption-free solid-phase extraction of surface

metabolites from macroalgae

Emilio Cirri and Georg Pohnert

Section III: Cellular and molecular characterization

Chapter 21 The immunodetection and in situ imaging of

cell-wall polysaccharides in brown algae

Amandine Siméon, Delphine Duffieux, Cécile Hervé,

Sophie Le Panse, Paul Knox, and Thomas Torode

Chapter 22 Atomic force microscopy based analysis of cell-wall

elasticity in macroalgae

Thomas Torode, Marina Linardic, J. Louis Kaplan,

and Siobhan A. Braybrook

Chapter 23 Dynamic and microscale mapping of cell growth:

Case of Ectocarpus filament cells

Hervé Rabillé, Bernard Billoud, Elodie Rolland,

and Bénédicte Charrier

Chapter 24 Actin fluorescent staining in the filamentous brown

alga Ectocarpus siliculosus

Hervé Rabillé, Maria Koutalianou, Bénédicte Charrier,

and Christos Katsaros

Chapter 25 Cryofixation of brown algae for transmission

electron microscopy

Chikako Nagasato, Christos Katsaros, and Taizo Motomura

Chapter 26 Probing the subcellular topography of

seaweeds: Transmission electron microscopy,

immunocytochemistry, and correlative light


Sandra C. Raimundo and David S. Domozych

Chapter 27 Coralline algae preparation for scanning electron

microscopy and optical microscopy

S. Kaleb, G. Alongi, and A. Falace

Chapter 28 Extraction of high quality RNA from brown algae

for transcriptomic analysis

Sandra Heinrich

Chapter 29 Induction of sexual reproduction in Spirogyra

cultures for laser capture microdissection of gametes

and zygotes

Denis Saint-Marcoux and Jane A. Langdale

Chapter 30 Cloning and expression strategies for the

postgenomic analysis of brown algae

Agnès Groisillier

Chapter 31 Polyethylene glycol-mediated transformation in

the green macroalga Ulva mutabilis (Chlorophyta):

A forward genetics approach

Jens Boesger, Michiel Kwantes, and Thomas Wichard


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