Radical SAM Enzymes

by Vahe Bandarian (Editor)

Overview

Radical SAM Enzymes, Volume 606, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on the Characterization of the glycyl radical enzyme choline trimethylamine-lyase and its radical S-adenosylmethionine activating enzyme, Diphathimide biosynthesis, Radical SAM glycyl radical activating enzymes, Radical SAM enzyme BioB in the biosynthesis of biotin, Biogenesis of the PQQ cofactor, Role of MoaAC in the biogenesis of the molybdenum cofactor, Biosynthesis of the nitrogenase cofactor, Bioinformatics of the radical SAM superfamily, The involvement of SAM radical enzymes in the biosynthesis of methanogenic coenzymes, methanopterin and coenzyme F420, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Covers radical SAN enzymes in detail

Product Details

ISBN-13:	9780128127957
Publisher:	Elsevier Science & Technology Books
Publication date:	08/08/2018
Series:	Methods in Enzymology , #606
Sold by:	Barnes & Noble
Format:	eBook
Pages:	538
File size:	57 MB
Note:	This product may take a few minutes to download.

About the Author

Vahe Bandarian (b. 1970) received his BS degree in Biochemistry from the California State University in Los Angeles in 1992 working with Scott Grover on kinetic studies of phosphoenolpyruvate carboxylase. He received his PhD in Biochemistry in 1998 from the University of Wisconsin-Madison working under the direction of George Reed carrying out electron paramagnetic studies of the cobalamin-dependent enzyme ethanolamine ammonia-lyase. After carrying out postdoctoral studies with Rowena Matthews on the cobalamin-dependnet methionine synthase at the University of Michigan in Ann Arbor he joined the faculty of the Department of Biochemistry at the University of Arizona in 2003. In 2015 he moved as Professor of Chemistry to the University of Utah. Vahe’s lab studies biosynthetic pathways to natural products that include modified nucleic acids and peptides. His contributions have been recognized by a Burroughs Wellcome Career Award in Biomedical Sciences and the Pfizer Award in Enzyme Chemistry by the Division of Biological Chemistry at ACS.

Table of Contents

1. Atlas of the Radical SAM Superfamily: Divergent Evolution of Function Using a "Plug and Play" DomainGemma L. Holliday, Eyal Akiva, Elaine C. Meng, Shoshana D. Brown, Sara Calhoun, Ursula Pieper, Andrej Sali, Squire J. Booker and Patricia C. Babbitt2. Purification and Characterization of the Choline Trimethylamine-Lyase (CutC)-Activating Protein CutDSmaranda Bodea and Emily P. Balskus3. QueE: A Radical SAM Enzyme Involved in the Biosynthesis of 7-Deazapurine Containing Natural ProductsJulia K. Lewis, Nathan A. Bruender and Vahe Bandarian4. TYW1: A Radical SAM Enzyme Involved in the Biosynthesis of Wybutosine BasesAnthony P. Young and Vahe Bandarian5. Mechanistic Studies on the Radical SAM Enzyme Tryptophan Lyase (NosL)Dhananjay M. Bhandari, Dmytro Fedoseyenko and Tadhg P. Begley6. Aminofutalosine Synthase (MqnE): A New Catalytic Motif in Radical SAM EnzymologySumedh Joshi, Dmytro Fedoseyenko, Nilkamal Mahanta and Tadhg P. Begley7. Iterative Methylations Resulting in the Biosynthesis of the t-Butyl Group Catalyzed by a B12-Dependent Radical SAM Enzyme in Cystobactamid BiosynthesisYuanyou Wang, Bastien Schnell, Rolf Müller and Tadhg P. Begley8. Biochemical Approaches for Understanding Iron-sulfur Cluster Regeneration in Escherichia coli Lipoyl Synthase during CatalysisErin L. McCarthy and Squire J. Booker9. Using Peptide Mimics to Study the Biosynthesis of the Side-Ring System of NosiheptideBo Wang, Joseph W. LaMattina, Edward D. Badding, Lauren K. Gadsby, Tyler L. Grove and Squire J. Booker10. Mechanistic Studies of Radical SAM Enzymes: Pyruvate Formate-Lyase Activating Enzyme and Lysine 2,3-Aminomutase Case StudiesAmanda S. Byer, Elizabeth C. McDaniel, Stella Impano, William E. Broderick and Joan B. Broderick11. Determining Redox Potentials of the Iron–Sulfur Clusters of the AdoMet Radical Enzyme SuperfamilyStephanie J. Maiocco, Lindsey M. Walker and Sean J. Elliott12. Radical S-Adenosyl-L-Methionine (SAM) Enzyme Involved in the Maturation of the Nitrogenase ClusterLee Rettberg, Kazuki Tanifuji, Andrew Jasniewski, Markus Walter Ribbe and Yilin Hu13. Purification, Characterization, and Biochemical Assays of Biotin Synthase from Escherichia coliJulia D. Cramer and Joseph T. Jarrett14. Methods for Expression, Purification, and Characterization of PqqE, a Radical SAM Enzyme in the PQQ Biosynthetic PathwayWen Zhu, Ana M. Martins and Judith P. Klinman15. Methods for Studying the Radical SAM Enzymes in Diphthamide BiosynthesisMin Dong, Yugang Zhang and Hening Lin16. Guidelines for Determining the Structures of Radical SAM Enzyme-Catalyzed Modifications in the Biosynthesis of RiPP Natural ProductsLeah B. Bushin and Mohammad R. Seyedsayamdost17. Identification of the Radical SAM Enzymes Involved in the Biosynthesis of Methanopterin and Coenzyme F420 in MethanogensKylie D. Allen and Robert H. White18. Lessons From the Studies of a C-C Bond Forming Radical SAM Enzyme in Molybdenum Cofactor BiosynthesisHaoran Pang and Kenichi Yokoyama

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From the Publisher

The volume brings together range of practical methods for dealing with all types of radical SAM enzymes, from bioinformatics, to protein purification and characterization