Regulators and Effectors of Small GTPases, Part E: GTPases Involved in Vesicular Traffic

by John N. Abelson (Editor), Melvin I. Simon (Editor), W. E. Balch (Editor), Channing J. Der (Editor), Alan Hall (Editor)

Overview

Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport.This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.

Product Details

ISBN-13:	9780080496849
Publisher:	Elsevier Science & Technology Books
Publication date:	02/08/2001
Series:	Methods in Enzymology , #329
Sold by:	Barnes & Noble
Format:	eBook
Pages:	553
File size:	10 MB

Table of Contents

Section I. Rab GTPases 1: Structural Basis for Rab Function: An Overview 2: Expression of Wild-Type and Mutant Green Fluorescent Protein-Rabl for Fluorescence Microscopy Analysis 3: Fluorescence Methods for Monitoring Interactions of Rab Proteins with Nucleotides, Rab Escort Protein, and Geranylgeranyltransferase 4: Prenylation of Rab Proteins in Vitro by Geranylgeranyltransferases 5: Antibody and Oligonucleotide Probes to Distinguish Intracellular Expression and Localization Patterns of Rab GDP-Dissociation Inhibitor Isoforms 6: Expression, Purification, and Biochemical Properties of Ypt/Rab GTPase-Activating Proteins of Gyp Family 7: Purification and Properties of Rab3 GDP/GTP Exchange Protein 8: Purification and Properties of Rab3 GTPase-Activating Protein 9: Rabphilin-3: A Target Molecule for Rab3 Small G Proteins 10: Doc2α as Modulator of Ca2+-Dependent Exocytosis 11: Purification and Properties of a GTPase-Activating Protein for Yeast Rab GTPases 12: Purification and Characterization of Yeast Exocyst Complex 13: Expression and Properties of Rab4 and Its Effector Rabaptin-4 in Endocytic Recycling 14: Purification of EEA1 from Bovine Brain Cytosol Using Rab5 Affinity Chromatography and Activity Assays 15: Expression, Purification, and Characterization of Rab5 Effector Complex, Rabaptin-5/Rabex-5 16: Measurement of Rab5 Protein Kinase B/akt and Regulation of Ras-Activated Endocytosis 17: Expression, Purification, and Biochemical Properties of Rabkinesin-6 Domains and Their Interactions with Rab6A 18: Expression and Functional Analyses of Rab8 and Rablla in Exocytic Transport from trans-Golgi Network 19: Expression and Properties of Rab7 in Endosome Function 20: Expression, Purification, and Properties of Rab8 Function in Actin Cortical Skeleton Organization and Polarized Transport 21: Properties of Rab13 Interaction with Rod cGMP Phosphodiesterase δ Subunit 22: Subcellular Localization of Rab17 by Cryo-Immunogold Electron Microscopy in Epithelial Cells Grown on Polycarbonate Filters 23: Expression and Properties of Rab25 in Polarized Madin-Darby Canine Kidney Cells 24: Purification of TRAPP from Saccharomyces cerevisiae and Identification of Its Mammalian Counterpart Section II.