Rhodopsin: Methods and Protocols

This volume explores the technological improvements in protein engineering, expression, purification, and crystallization of several rhodopsin photoactive intermediates, thus increasing our understanding of rhodopsin activation. The first chapters of the book focus on methods developed to study fundamentals of rhodopsin structure and function, starting with improved purification prools of native and mutated rhodopsin, followed by methods used for rhodopsin reconstitution into lipid bilayers...