Synthetic mRNA: Production, Introduction Into Cells, and Physiological Consequences

This volume presents detailed laboratory prools for in vitro synthesis of mRNA with favorable properties, its introduction into cells by a variety of techniques, and the measurement of physiological and clinical consequences such as protein replacement and cancer immunotherapy. Synthetic techniques are described for structural features in mRNA that provide investigational tools such as fluorescence emission, click chemistry, photo—chemical crosslinking, and that produce mRNA with increased ...