Techniques in Protein Chemistry III

Techniques in Protein Chemistry III

by Ruth Hogue Angeletti

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Overview

Techniques in Protein Chemistry III by Ruth Hogue Angeletti

Techniques in Protein Chemistry III compiles papers presented at the Fifth Protein Society Symposium in Baltimore on June 22-26, 1991.
This book discusses the protein and peptide recovery from PVDF membranes; high-sensitivity peptide mapping utilizing reversed-phase microbore and microcolumn liquid chromatography; and capillary electrophoresis for preparation of peptides and direct determination of amino acids. The TFMSA/TFA cleavage in t-Boc peptide synthesis; applications of automatic PTC amino acid analysis; and identification of O-glycosylation sites with a gas phase sequencer are also elaborated. This text likewise covers the conformational stability of the molten globule of cytochrome c and role of aqueous solvation in protein folding.
This publication is useful to students and researchers interested in methods and research approaches on protein chemistry.

Product Details

ISBN-13: 9781483259468
Publisher: Elsevier Science
Publication date: 06/28/2014
Sold by: Barnes & Noble
Format: NOOK Book
Pages: 572
File size: 13 MB
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Table of Contents

Foreword


Preface


Acknowledgments


Section I Protein Microsequence Methods


Protein and Peptide Recovery from PVDF Membranes


Automated C-Terminal Sequencing of Peptides


Elution and Internal Amino Acid Sequencing of PVDF-Blotted Proteins


Evaluation of Protein Sequencing Core Facilities: Design, Characterization, and Results from a Test Sample (ABRF-91SEQ)


Optimization of Solid Phase Peptide Sequencing


Evaluation of the Blott Cartridge for Enhanced Gas Phase Sequencing at Maximum Sensitivity


Reusing PVDF Electroblotted Protein Samples After N-Terminal Sequencing to Obtain Unique Internal Amino Acid Sequence


Chromatographic Carbon as an Inert Sample Adsorbent for Protein Sequencing


Na-Acylaminoacyl-Peptide Hydrolase: Specificity and Use toUnblock N-Acetylated Proteins


Section II Protein Microsequencing Workshop


Strategies for the Isolation of Peptides from Low-Abundance Proteins for Internal Sequence Analysis


High-Sensitivity Peptide Mapping Utilizing Reversed-Phase Microbore and Microcolumn Liquid Chromatography


Microscale Protein Isolation and Microsequencing Workshop: An Overview


Section IIΙ Peptide/Protein Separation Techniques


Micropreparative Separation of Tryptic Digests by Capillary Electrophoresis and Characterization by Protein Sequencing


Estimation of Protein Isoelectric Points by Capillary Electrophoresis Using MICRO-COAT™


Capillary Electrophoresis for Preparation of Peptides and Direct Determination of Amino Acids


Perfusion Chromatography: A Novel Tool for Protein Purification and Analysis


Comparison of Coupling Procedures for Development of Affinity Membranes: Optimization of the CDI Method


Section IV Peptide Synthesis


Randomization of Amino Acid Sequence: An Important Control in Synthetic Peptide Analogue Studies of Nucleic Acid Binding Domains


Comparative Analysis of Synthetic Peptides by Free-Solution Capillary Electrophoresis (FSCE) and Strong-Cation Exchange (SCX)-HPLC


Immunoreactive Synthetic Peptide Epitope Mapping with Structural Validation Using Electrospray Mass Spectrometry


TFMSA/TFA Cleavage in t-Boc Peptide Synthesis


Synergy of Semisynthesis and Site-Directed Mutagenesis:


Manipulating Genes to Create Sites for Autocatalytic Protein Fragment Religation


State-of-the-Art Peptide Synthesis: Comparative Characterization of a 16-mer Synthesized in 31 Different Laboratories


Section V Amino Acid Analysis


Applications of Automatic PTC Amino Acid Analysis


LC-Determination of D- and L-Amino Acids in Peptide Hydrolysates by Pre-column Derivatization with a Chiral Reagent


Amino Acid Analysis of PVDF Bound Proteins


Collaborative Trial Analyses of ABRF-91AAA


Section VI Structural Analysis of Glycoproteins


Identification of O-Glycosylation Sites with a Gas Phase Sequencer


Glycoform, Glycotype Linkage, and Branching Detail of the CD2 Adhesion Domain by Desolvation Mass Spectrometry: SFCI-MS and ESI-MS


Characterization of the Glycosylation on Recombinant Human Low-Affinity Nerve Growth Factor Receptor


Nonenzymic Glycation of Hemoglobin: Structural Determinants of Site Selectivity


A Unified Approach to Glycoprotein Primary Structure Analysis: Identification, Isolation, and Characterization of Both Peptide and Pendant Carbohydrate of Glycopeptides


Section VII Protein Folding and Conformation


Refolding of Human Recombinant Insulin-like Growth Factor II (IGF-II) in Vitro, Using a Solubilizing Affinity Handle


Conformational Stability of the Molten Globule of Cytochrome C: Role of Electrostatic Repulsion


Calcium-Induced Folding of Troponin-C: Formation of Homodimeric and Heterodimeric Two-Site Domains from Synthetic Peptides


The Role of Aqueous Solvation in Protein Folding: Insights from Aqueous-Alkane Partitioning


Molecular Characterization of an Aggregate Formed by a Bovine Growth Hormone Folding Intermediate


Use of Sequence Hydrophobic Moment to Analyze Membrane Interacting Domains of Butulinum, Tetanus, and Other Toxins


Fourier Transform Infrared Spectroscopic Analysis of Proteins in Terms of Detectability, Conformation, and Surface Adsorption Density


Recombinant Hemoglobins for the Elucidation of a New Mechanism of Oxygen Affinity Modulation by Cl-Ions


An Improved Strategy for the Determination of the Role in Catalysis of Amino Acid Residues in Thymidylate Synthase


Protein Conformational Changes in the μ& Time Region Investigated with a Laser Pulse Photolysis Technique


Macromolecular Dynamics Observed by Intramolecular Energy Transfer Using Frequency-Domain Fluorometry


Stopped-Flow Circular Dichroism and 19F NMR as Probes for the Folding of Rat Intestinal Fatty-Acid Binding Protein (IFABP)


Section VIII Mass Spectrometry


Analysis of Protein-Protein Binding with Laser Desorption Mass Spectrometry: Can Multimeric Forms Be Studied?


Applications of Liquid Chromatography-Electrospray Mass Spectrometry (LC-ES/MS)


Analysis of Proteins by Mass Spectrometry


Characterization of PEGated Superoxide Dismutase


Ν-ε-Acetylation Can Occur at Lysine Residues 157,167,171, and 180 of Recombinant Bovine Somatotropin


Plasma Desporption Mass Spectral (PDMS) Analysis of Polypeptides Containing Some Post-translational Modifications; Scission of Covalent Bonds


Determination of the Cleavage Site of the Amyloid Precursor Protein by Plasma Desorption Mass Spectrometry 505


Large-Scale Protein Mapping Using Infrequent Cleavage Reagents, LD TOF MS, and ES MS


Tandem Mass Spectrometric Sequencing of Proteins Isolated from 1- and 2-Dimensional Polyacrylamide Gel Electrophoresis


Index



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