The Hepatocyte Review

It is thirty years since the technique of high-yield preparation of isolated hepaytes, by collagenase perfusion of the liver, was published. The original method described by Berry and Friend has undergone many minor modifications by other workers, and the two-step procedure introduced by Seglen in 1976 has become the most frequent way to prepare hepayte suspensions. An important development introduced by Bissell in 1973 was the use of the cells as the first step in monolayer culture. The av...