ADP-ribosylation and NAD+ Utilizing Enzymes: Methods and Protocols

This volume focuses on mono—ADP—ribosylation and enzymes that use NAD+ including Sirtuins, PARPs, and bacterial and eukaryotic ADP—ribosyltransferases. The chapters in this book are organized into eight parts, and offer detailed descriptions of key prools used to study topics such as in vitro techniques for ADP—ribosylation substrate identification; biochemical and biophysical assays of PAR—WWE domain interactions; monitoring expression and enzyme activity of ecto—ARTCs; HPLC—based enzymes ...