Caspases,Paracaspases, and Metacaspases: Methods and Protocols

Caspases,Paracaspases, and Metacaspases: Methods and Protocols

Paperback(Softcover reprint of the original 1st ed. 2014)

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Product Details

ISBN-13: 9781493954056
Publisher: Springer New York
Publication date: 08/23/2016
Series: Methods in Molecular Biology , #1133
Edition description: Softcover reprint of the original 1st ed. 2014
Pages: 266
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

Part I: Caspases

1. General in vitro Caspase Assay Procedures

Dave Boucher, Catherine Duclos, Jean-Bernard Denault

2. Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Specificity

Marcin Poręba, Aleksandra Stróżyk, Paulina Kasperkiewicz, Marcin Drąg

3. Global Identification of Caspase Substrates using PROTOMAP (PROtein TOpography and Migration Analysis Platform)

Melissa M. Dix, Gabriel M. Simon, Benjamin F. Cravatt

4. Caspase-2 Protocols

Loretta Dorstyn and Sharad Kumar

5. Caspase-14 Protocols

Mami Yamamoto-Tanaka and Toshihiko Hibino

6. Caspase Protocols in Caenorhabditis elegans

Eui Seung Lee & Ding Xue

7. Detecting Caspase Activity in Drosophila Larval Imaginal Discs

Caitlin E. Fogarty and Andreas Bergmann

8. Methods for the Study of Caspase Activation in the Xenopus laevis Oocyte and Egg Extract

Francis McCoy, Rashid Darbandi, and Leta K. Nutt

9. Caspase Protocols in Mice

Varsha Kaushal, Christian Herzog, Randy S. Haun, and Gur P. Kaushal

10. Measurement of Caspase Activation in Mammalian Cell Cultures

Magnus Olsson and Boris Zhivotovsky

Part II: Paracaspases and Metacaspases

11. Detection and Measurement of Paracaspase MALT1 Activity

Stephan Hailfinger, Christiane Pelzer, and Margot Thome

12. Leishmania Metacaspase: an Arginine-Specific Peptidase

Ricardo Martin, Iveth Gonzalez and Nicolas Fasel

13. Purification, Characterization, and Crystallisation of Trypanosoma Metacaspases
Karen McLuskey, Catherine X. Moss, and Jeremy C. Mottram

14. Monitoring the Proteostasis Function of the Saccharomyces cerevisiae Metacaspase Yca1

Amit Shrestha, Robin E. C. Lee, and Lynn A. Megeney

15. Plant Metacaspase Activation and Activity

Elena A. Minina, Simon Stael, Frank Van Breusegem, and Peter V. Bozhkov

16. Preparation of Arabidopsis thaliana Seedling Proteomes for Identifying Metacaspase Substrates by N-terminal COFRADIC

Liana Tsiatsiani, Simon Stael, Petra Van Damme, Frank Van Breusegem, and Kris Gevaert

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