Endocannabinoid Signaling: Methods and Protocols

by Mauro Maccarrone (Editor)

Overview

This second edition provides new and updated chapters detailing all major elements of the ECB system. Chapters guide readers through identification of drug targets, electrophysiology, computational chemistry, and machine learning. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Endocannabinoid Signaling: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about this important and developing field.

Product Details

ISBN-13:	9781071627303
Publisher:	Springer US
Publication date:	09/25/2023
Series:	Methods in Molecular Biology , #2576
Edition description:	2nd ed. 2023
Pages:	509
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Need for Methods to Investigate Endocannabinoid Signaling.- Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues.- Determination of 2-Arachidonoylglycerol by µSPE-LC-MS/MS.- Analysis of Omega-3 Fatty Acid Derived N-acylethanolamines in Biological Matrices.- Assay of CB₁ Receptor Binding.- The Displacement Binding Assay Using Human Cannabinoid CB₂ Receptor-Transfected Cells.- Assay of TRPV1 Receptor Signaling.- A Functional Assay for GPR55: Envision Prools.- The Cyclic AMP Assay Using Human Cannabinoid CB₂ Receptor-Transfected Cells.- Assay of GTPγS Binding in Autoradiography.- Prool to Study β-Arrestin Recruitment by CB₁ and CB₂ Cannabinoid Receptors.- Assay of NAT Activity.- Assay of NAPE-PLD Activity.- Assay of FAAH Activity.- Assay of NAAA Activity.- Assay of DAGLα/β Activity.- Assay of Monoacylglycerol Lipase Activity.- A Sensitive and Versatile Fluorescent Activity Assay for ABHD6.- A Sensitive and Versatile Fluorescent Activity Assay for ABHD12.- Assay of Endocannabinoid Uptake.- Assay of Endocannabinoid Oxidation by Cyclooxygenase-2.- Oxygenation of Anandamide by Lipoxygenases.- Assay of Endocannabinoid Oxidation by Cyhrome P_450.- Assessing Gene Expression of the Endocannabinoid System.- Western Blotting of the Endocannabinoid System.- Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells.- Measuring ECS Interaction with Biomembranes.- Visualization of Endocannabinoids in the Cell.