Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008.
Fluorescent Proteins II highlights the physicochemical and biophysical aspects of fluorescent protein technology beyond imaging. It is tailored to meet the needs of physicists, chemists and biologists who are interested in the fundamental properties of fluorescent proteins, while also focussing on specific applications. The implementations described are cutting-edge studies and exemplify how the physical and chemical properties of fluorescent proteins can stimulate novel findings in life sciences.
Table of Contents
Part I. Fluorescence Microscopy beyond Imaging
· Fluorescent Proteins: Nature’s Colorful Gifts for Live Cell Imaging (Jörg Wiedenmann)
· Green Fluorescent Protein Photodynamics as a Tool for Fluorescence Correlative Studies and Applications (Giuseppe Chirico)
Part II. Quantification of Basic Physiological Parameters
· The proton sensitivity of Fluorescent Proteins: towards intracellular pH indicators (Ranieri Bizzari)
· Green Fluorescent Protein-Based Chloride Ion Sensors for In Vivo Imaging (Daniele Arosio)
· Fluorescent Genetically Encoded Calcium Indicators and Their In Vivo Application (Thomas Gensch)
· Action Potentials in Heart Cells (Lars Kaestner)
Part III. Advanced Bioanalytical Applications
· Probing Structure and Dynamics of the Cell Membrane with Single Fluorescent Proteins (Thomas Schmidt)
· Fluorescence Correlation and Cross-Correlation Spectroscopy Using Fluorescent Proteins for Measurements of Biomolecular Processes in Living Organisms (Thorsten Wohland)
· Investigating the lifecycle of HIV with fluorescent proteins (Don Lamb, Barbara Müller)