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G Protein-Coupled Receptor Signaling: Methods and Protocols

G Protein-Coupled Receptor Signaling: Methods and Protocols

by Mario Tiberi (Editor)

Hardcover(1st ed. 2019)

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Available for Pre-Order. This item will be available on March 1, 2019

Product Details

ISBN-13: 9781493991204
Publisher: Springer New York
Publication date: 03/01/2019
Series: Methods in Molecular Biology , #1947
Edition description: 1st ed. 2019
Pages: 377
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

Part I: Probing Structural Properties of GPCRs



1. Assessment of Conformational State Transitions of Class B GPCRs Using Molecular Dynamics


Chenyi Liao, Victor May, and Jianing Li



2. Molecular Dynamic Simulations to Probe Water Permeation Pathways of GPCRs


Katsufumi Tomobe, Eiji Yamamoto, and Kenji Yasuoka



3. Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies



Fabian Bumbak, Ross A.D. Bathgate, Daniel J. Scott, and Paul R. Gooley



4. A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties


Anne Zemella, Theresa Richter, Lena Thoring, and Stefan Kubick



Part II: Tweaking Ligands for GPCR Studies



5. Furan Cross-Linking Technology for Investigating GPCR-Ligand Interactions


Marleen Van Troys, Willem Vannecke, Christophe Ampe, and Annemieke Madder



6. Optical Regulation of Class C GPCRs by Photoswitchable Orthogonal Remotely-Tethered Ligands


Amanda Acosta-Ruiz, Johannes Broichhagen, and Joshua Levitz



7. Chemoselective Acylation of Hydrazinopeptides to Access Fluorescent Probes for Time-Resolved FRET Assay on GPCRs


Sridévi M. Ramanoudjame, Lucie Esteoulle, Stéphanie Riché, Jean-François Margathe, Thierry Durroux, Iuliia A. Karpenko, and Dominique Bonnet



Part III: Bioluminescence and Fluorescence Resonance Transfer Energy Approaches



8. Time-Resolved FRET-Based Assays to Characterize G Protein-Coupled Receptor Hetero-Oligomer Pharmacology


Joyce Heuninck, Candide Hounsou, Elodie Dupuis, Eric Trinquet, Bernard Mouillac, Jean-Philippe Pin, Dominique Bonnet, and Thierry Durroux



9. Combining Conformational Profiling of GPCRs with CRISPR/Cas9 Gene Editing Approaches


Kyla Bourque, Dominic Devost, Asuka Inoue, and Terence E. Hébert



10. Measuring GPCR Stoichiometry Using Types -1, -2 and -3 Bioluminescence Resonance Energy Transfer-Based Assays


James H. Felce, John R. James, and Simon J. Davis



11. Combining SRET2 and BiFC to Study GPCR Heteromerization and Protein-Protein Interactions


Amina M. Bagher, Melanie E.M. Kelly, and Eileen M. Denovan-Wright



12. Quantification and Comparison of Signals Generated by Different FRET-Based cAMP Reporters


Andreas Koschinski and Manuela Zaccolo



Part IV: Exploring GPCR Signaling Properties



13. Measuring GPCR-Induced Activation of Protein Tyrosine Phosphatases (PTP) Using In-Gel and Colorimetric PTP Assays


Geneviève Hamel-Côté, Fanny Lapointe, and Jana Stankova



14. Measurement of β-Arrestin Recruitment at GPCRs Using the Tango Assay


Geneviève Laroche and Patrick M. Giguère



15. Probing the Interactome of Corticotropin-Releasing Factor Receptor Heteromers Using Mass Spectrometry


Burcu Hasdemir, Juan A. Oses-Prieto, Alma Burlingame, and Aditi Bhargava



Part V: Visualizing Subcellular Compartmentalization of GPCRs



16. Monitoring the Aggregation of GPCRs by Fluorescence Microscopy


Samuel Génier, Jade Degrandmaison, Christine L. Lavoie, Louis Gendron, and Jean-Luc Parent



17. Combining RNAi and Immunofluorescence Approaches to Investigate Post-Endocytic Sorting of GPCRs into Multivesicular Bodies


Xuezhi Li, Stéphanie Rosciglione, Andréanne Laniel, and Christine Lavoie



18. Super-Resolution Imaging of G Protein-Coupled Receptors Using Ground State Depletion Microscopy


Fabiana A. Cateano-Crowley, Bryan Heit, and Stephen S.G. Ferguson



19. Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy


Kim C. Jonas and Aylin C. Hanyaloglu



Part VI: Investigating In Vivo Functions of GPCRs



20. Optical Modulation of Metabotropic Glutamate Receptor Type 5 In Vivo Using a Photoactive Drug


Marc López-Cano, Joan Font, Amadeu Llebaria, Víctor Fernández-Dueñas, and Francisco Ciruela



21. Imaging of Tissue-Specific and Temporal Activation of GPCR Signaling Using DREADD Knock-In Mice


Dmitry Akhmedov, Nicholas S. Kirkby, Jane A. Mitchell, and Rebecca Berdeaux



22. Laser Doppler Flowmetry to Study the Regulation of Cerebral Blood Flow by G Protein-Coupled Receptors in Rodents


Xavier Toussay, Mario Tiberi, and Baptiste Lacoste



23. Preparation of Pulmonary Artery Myocytes and Rings to Study Vasoactive GPCRs


Martha Hinton, Anurag Singh Sikarwar, and Shyamala Dakshinamurti